We narrowed to 8,768 results for: PROC

1. AAV-GFP/Cre

   Plasmid

   #49056

   Purpose

   Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

   Depositor

   Fred Gage

   Article

   Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

   Insert

   Cre
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP, Myc, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SARS-CoV-2 5'UTR-Luciferase

   Plasmid

   #191480

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   SARS-CoV-2 5' UTR
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   contains SARS-CoV-2 5' UTR

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBV-Luc mut MBS1-4

   Plasmid

   #16565

   Depositor

   Bert Vogelstein

   Article

   Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

   Insert

   c-MYC binding sites (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant MBS1-4

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA6-N-Myc-SCAP

   Plasmid

   #134285

   Purpose

   Expresses Myc-tagged SCAP in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SCAP (Scap, Scap Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-DUET1-DHDDS/Sumo-NgBR

   Plasmid

   #203162

   Purpose

   Expression of human cis-prenyltransferase complex for purification

   Depositor

   William Sessa

   Article

   Edani et al Proc Natl Acad Sci U S A. 2020 Aug 25;11

   Inserts

   DHDDS (DHDDS Human)
   NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1 Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   truncation

   Promoter

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER11 (miR-RUG)

   Plasmid

   #44363

   Purpose

   Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

   Depositor

   Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Insert

   PheS Gly294
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6-N-Flag-SREBP2

   Plasmid

   #134284

   Purpose

   Expresses Flag-tagged SREBP2 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP2 (Srebf2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP2 precursor

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_EF1a_Flag_TP53_WT

   Plasmid

   #183112

   Purpose

   Expresses flag-tagged p53 in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   EF1a

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherryC2-Gephyrin P1

   Plasmid

   #68820

   Purpose

   mCherry tagged Geph for mammalian expression

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyrin…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. IL6R PLAK1

    Plasmid

    #161518

    Purpose

    Expresses IL6 receptor (full length) in mammalian cells.

    Depositor

    Yoav Shaul

    Article

    Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

    Insert

    Interleukin 6 Receptor (IL6R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid

    #106309

    Purpose

    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    

    Use

    Tags

    6xflag-3xHaloTag(MtoL)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTreTight-Htt94Q-CFP

    Plasmid

    #23966

    Depositor

    Nico Dantuma

    Article

    Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

    Insert

    N-terminal fragment Huntingtin Q94 (HTT Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    94 polyQ repeat

    Promoter

    Available since

    April 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_EF1a_Flag_TP53_R248W+P278A

    Plasmid

    #183115

    Purpose

    Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed proline 278 to alanine and arginine 248 t…

    Promoter

    EF1a

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. hOT-GFP

    Plasmid

    #67848

    Purpose

    mammalian expression of human oxytocin receptor with GFP tag

    Depositor

    Christian Gruber

    Article

    Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

    Insert

    oxytocin receptor (OXTR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. VN-SOD1

    Plasmid

    #89757

    Purpose

    expresses human SOD1 fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_EF1a_Flag_TP53_R248W

    Plasmid

    #183114

    Purpose

    Expresses flag-tagged p53 with R248W mutation in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 248 to tryptophan

    Promoter

    EF1a

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SOD1-VC

    Plasmid

    #89758

    Purpose

    expresses human SOD1 fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG104 FAST

    Plasmid

    #130720

    Purpose

    Expresses FAST (also called YFAST) in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    FAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSicoR-Ef1a-mCh-Puro

    Plasmid

    #31845

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. FKBP-mEGFP-YAP-MAML2

    Plasmid

    #217612

    Purpose

    part A of YAP-MAML2 SPARK-OFF

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    FKBP-mEGFP-YAP-MAML2 (YAP1, MAML2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA5/avitag-apex2-v5-fkbp8

    Plasmid

    #154460

    Purpose

    expresses avitag-apex2-v5-fkbp8/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    avitag-apex2-v5-fkbp8 (FKBP8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F, T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCEP4-FLAG-TAPBPR-TM-VC

    Plasmid

    #135499

    Purpose

    Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    Luminal/Extracellular FLAG epitope tag, Signal pe…

    Expression

    Mammalian

    Mutation

    switched transmembrane domain with that of MHC-I

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. t1-435

    Plasmid

    #166578

    Purpose

    For expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

    Insert

    Tln1 Head (1-435) (TLN1 Human)
    

    Use

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-435 only

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MSCV-Tox-IRES-eGFP

    Plasmid

    #165427

    Purpose

    Expresses TOX in mammalian cells

    Depositor

    Patrick Hogan, Anjana Rao

    Article

    Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

    Insert

    TOX (Tox Mouse)
    

    Use

    Tags

    3xflag 5' of Tox

    Expression

    Mammalian

    Mutation

    Promoter

    5' LTR

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

    Plasmid

    #166970

    Purpose

    Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.

    Depositor

    Matthew Banghart

    Article

    Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG, T2A, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAD29_hIRG1_4-461_pvp008

    Plasmid

    #124843

    Purpose

    Expresses human CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 462-end.

    Promoter

    T7

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MigR1-AE

    Plasmid

    #12431

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

    Insert

    AML1-ETO fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YFP NLS Beta-Actin G13R

    Plasmid

    #60615

    Purpose

    Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    G13R

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. eGFP-proα2(I)

    Plasmid

    #119826

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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