We narrowed to 17,982 results for: ERG

1. pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA

   Plasmid

   #108798

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

   Plasmid

   #127868

   Purpose

   pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1 (Nos1 Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Mutation

   Amino acids 1-133 only

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA

   Plasmid

   #108805

   Purpose

   Expresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa233
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA

   Plasmid

   #108806

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA

   Plasmid

   #108795

   Purpose

   Expresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa571
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA

   Plasmid

   #108796

   Purpose

   Expresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa572 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FKBP and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSL0747 (pDonor_L-MmeI)

   Plasmid

   #130647

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (L*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATG --> TGTTGGAG

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HA-VHL C162F-pRc/CMV

   Plasmid

   #22042

   Depositor

   William Kaelin

   Article

   Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   point mutant, converting aa 162 from Cysteine to …

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSL0746 (pDonor_R-MmeI)

   Plasmid

   #130646

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (R*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATA --> TGTTGGAA

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

    Plasmid

    #108769

    Purpose

    Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

    Plasmid

    #127865

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Mutation

    deleted the c-terminal 20 amino acids

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-VHL 1-167-pRc/CMV

    Plasmid

    #22078

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deletion mutant covering only aa 1-167 of wt VHL …

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-VHL L158S-pRc/CMV

    Plasmid

    #22041

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    point mutant converting aa 162 from Cysteine to P…

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNA0304

    Plasmid

    #165612

    Purpose

    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    NotI site replaced the CAN1 spacer in parent vect…

    Promoter

    SNR52

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

    Plasmid

    #127866

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Mutation

    C-terminal 20 amino acids only

    Promoter

    hSyn

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. N-TAP A2A-YFP pcDNA3

    Plasmid

    #67852

    Purpose

    mammalian expression of A2A receptor with N terminal TAP tag and C terminal YFP fusion

    Depositor

    Christian Gruber

    Article

    Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

    Insert

    adenosine A2a receptor (ADORA2A Human)
    

    Tags

    TAP and YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGG426_UV5

    Plasmid

    #165608

    Purpose

    Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    D1135V, S1136R, D1332K and R1333G mutations in Sp…

    Promoter

    lacUV5 driving Cas9 VRKG and UV5 driving sgRNA

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

    Plasmid

    #108814

    Purpose

    Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    TP901 integrase fragment aa327 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMIR-CDH1 mutUTR

    Plasmid

    #25039

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    CDH1 mutant 3'UTR (CDH1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    substitution of 6nt within the miR-9 binding site…

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

20. FB007

    Plasmid

    #119705

    Purpose

    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Promoter

    gpdA

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge