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pcDNA3 HA c-Jun

Plasmid

#237333

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Publication

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

c-Jun (JUN Human)

Tags

HA

Expression

Mammalian

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLM1110_LEU2

Plasmid

#168460

Purpose

Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

Depositor

Jef Boeke

Publication

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Insert

mRFP1

Use

Synthetic Biology; Yeast assembly vector for big-…

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXL-BacII-CAG-mCherry

Plasmid

#170519

Purpose

Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Publication

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-mCherry

Use

Piggybac transposon

Expression

Mammalian

Promoter

CAG

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_ADRB2-mTurquoise2-iLID

Plasmid

#161002

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Depositor

Sean R Collins

Publication

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

ADRB2-mTurquoise2-iLID

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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piRFP-FRB-PMP-C-10

Plasmid

#139316

Purpose

peroxisome-resident FRB for chemically induced dimerization

Depositor

Gerry Hammond

Publication

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

iRFP: MTOR(2021-2113):[GGSA]2QASNSAVSGLRSGSSGG:PXMP(2-195)

Expression

Mammalian

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h2

Plasmid

#86691

Purpose

For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Publication

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)

Tags

HA

Promoter

T3

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLVX-3XMyc-mTriml1

Plasmid

#51395

Depositor

Narry Kim

Publication

Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

Insert

Triml1 (Triml1 Mouse)

Use

Lentiviral

Tags

3XMyc

Expression

Mammalian

Promoter

EF1alpha

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1_sh_ex15

Plasmid

#35166

Depositor

Benjamin Blencowe

Publication

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
rGSKmyc-pXT7

Plasmid

#15492

Depositor

Sergei Sokol

Publication

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pStA212

Plasmid

#114171

Purpose

Start-Stop Assembly Level 212 plasmid

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Chicken ArcLight A173

Plasmid

#53615

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Publication

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-A173

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMYC BRGI aa1400-1648

Plasmid

#19145

Depositor

Joan Massague

Publication

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Tags

Myc

Expression

Mammalian

Mutation

human BRG1 aa 1400-1648 (PCR product)

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
BbZIP-cALFA

Plasmid

#160498

Purpose

For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

BbZIP

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Bacterial

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TAL2350

Plasmid

#36769

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

XPC-TALEN_Left (Xpc Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

Plasmid

#169097

Purpose

Cas9 and Citrine expression in transfected cells

Depositor

Marianne Bronner

Publication

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

Cas9-2A-Citrine

Expression

Mammalian

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4C

Plasmid

#202680

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Publication

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidC (608-773) (sidC Legionella pneumophila)

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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Drp1-EGFP-StrepII

Plasmid

#174429

Purpose

Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria

Depositor

Thomas Pucadyil

Publication

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

EGFP-StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRU293

Plasmid

#167688

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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