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pSFFV_sfCherry2_TagBFP

Plasmid

#83031

Purpose

Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry+full length_TagBFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

3 mutations in sfCherry full length: E118Q, T128I…

Promoter

SFFV

Available since

Jan. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PAsfCherry2(1-10)

Plasmid

#82612

Purpose

Express PAsfCherry2(1-10) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

PAsfCherry2(1-10)

Use

Tags

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

CMV

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRR-MR-5Z

Plasmid

#23059

Depositor

Charles Miller

Article

Miller et al Plasmid. 2009 Dec 3. ():.

Insert

MR (NR3C2 Human)

Use

Tags

Expression

Yeast

Mutation

N528H, Q814R, H993L

Promoter

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP no-pA

Plasmid

#80815

Purpose

nlsGFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-nlsGFP no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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ACS1

Plasmid

#13746

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Acetyl CoA Synthetase 1 (Acss2 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pICSL80005

Plasmid

#50322

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pME-FlEx switch

Plasmid

#82406

Purpose

Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP-T-CaURA3

Plasmid

#44877

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2(11)_TagBFP

Plasmid

#82606

Purpose

Expresses sfCherry2(11) and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_TagBFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

SFFV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJCSUP35

Plasmid

#1088

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

HIS tag

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2005

Availability

Academic Institutions and Nonprofits only
p5E-mnx1

Plasmid

#82402

Purpose

Gateway-compatible zebrafish mnx1 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mnx1 promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)

Plasmid

#72273

Purpose

FLAG-Mnase expressed under the REB1 promoter

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Tags

Expression

Mutation

Promoter

REB1

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pICH41421

Plasmid

#50339

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-GFP no-pA

Plasmid

#80826

Purpose

P2A followed by GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-GFP no-pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRSCupSup35GFP

Plasmid

#1087

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pICH41414

Plasmid

#50337

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Use

Tags

Gal4 activation domain

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pICSL12006

Plasmid

#50270

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5'UTR (Cassava Vein Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL30008

Plasmid

#50300

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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