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pKOdeltagalK (Hyg)

Plasmid

#110090

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx1

Plasmid

#158227

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx1

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

Plasmid

#50031

Purpose

Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

PM-SrtA(strep)

Tags

C-HA

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx2

Plasmid

#158225

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pJH291

Plasmid

#172153

Purpose

Reporter plasmid for pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.2TAG; Plpp pylS; PproK pylT; AraC

Expression

Bacterial

Mutation

sfGFP(N39*, Y151*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-iGECInano

Plasmid

#186189

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Bacterial

Promoter

AraC

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pARS3_MUbCAS9_MC

Plasmid

#128767

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA

Depositor

Zachary Nimchuk

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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XF1 GAL Smad3

Plasmid

#12635

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 (SMAD3 Human)

Tags

Gal4 DBD

Expression

Mammalian

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pCOND (Hyg)

Plasmid

#110093

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCYB-TnsA

Plasmid

#17356

Depositor

Nancy Craig

Article

Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

Insert

TnsA (tnsA E. coli transposon Tn7)

Tags

Intein-Chitin binding domain

Expression

Bacterial

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pGR-ECK120015170

Plasmid

#46006

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120015170 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pAC1819_pCR8-DR-SMN2-2-DR

Plasmid

#118653

Purpose

Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-SMN2-2-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM49981

Plasmid

#169087

Purpose

MoClo level 0 BvADHα coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvADHα

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Osh3

Plasmid

#58839

Purpose

Expresses GFP-Tagged Osh3 in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

Insert

Osh3p (OSH3 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
TRPML3-MYC

Plasmid

#18833

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pAGM921

Plasmid

#169086

Purpose

MoClo level 0 Db5GT coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Db5GT

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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DHFR

Plasmid

#64200

Purpose

Expression of E. coli DHFR

Depositor

Vern Schramm

Article

Wang et al J Am Chem Soc. 2014 Jun 11;136(23):8333-

Insert

Dihydrofolate reductase

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pLlac01

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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NK66

Plasmid

#176606

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

Syk-BFP (Syk Mouse, Synthetic)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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