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pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Publication

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Tags

Flag and GST

Expression

Bacterial

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pBoBi-hGOLGA2-C-PEN2

Plasmid

#183649

Purpose

Lentiviral expression of a golgi-residential PEN2.

Depositor

Sheng-cai Lin

Publication

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and GOLGA2 (PSENEN, GOLGA2 Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2+pEstrInd

Plasmid

#170880

Purpose

Phytobrick - Estradiol Inducible Promoter

Depositor

Markita Landry

Publication

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pEstradiol_Inducible

Expression

Plant

Mutation

c.837C>T and c.1353G>A to remove BsaI and E…

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Orf6

Plasmid

#157727

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Orf6 (ORF6 SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp12-HF

Plasmid

#157711

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PKAcs-GGSD5X-GFP

Plasmid

#108584

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Publication

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgMTHFD2_1

Plasmid

#106313

Purpose

Express Cas9 and sgRNA targeting MTHFD2

Depositor

Richard Possemato

Publication

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2 (MTHFD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mRuby2-roGFP1

Plasmid

#107219

Purpose

Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Publication

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

mRuby2-roGFP1

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo v2

Plasmid

#80033

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Publication

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MXS_CMV::HygroR-bGHpA

Plasmid

#62435

Purpose

MXS_chaining vector with CMV::HygroR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with CMV Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_MCS1

Plasmid

#62395

Purpose

MXS_chaining vector with MCS1

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Publication

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
pET28 Set9 mutant

Plasmid

#24083

Depositor

Danny Reinberg

Publication

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG and His

Expression

Bacterial

Mutation

H297A

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA1-Bystin

Plasmid

#11869

Depositor

Michiko Fukuda

Publication

Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

Insert

Bystin (BYSL Human)

Expression

Mammalian

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGMCS-0X-P38-PanBmGFP

Plasmid

#27127

Depositor

Sabine Ehrt

Publication

Gandotra et al PLoS Pathog. 2010 Aug 12;6(8):e1001040.

Insert

PanB

Tags

Linker (SGSGSG), GFPm2+

Expression

Bacterial

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pXP114

Plasmid

#26812

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Publication

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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SNAPtag-PTS1

Plasmid

#182883

Purpose

Express SNAPtag-PTS1 in mammalian cells. SNAPtag targets to peroxisomes

Depositor

Pekka Katajisto

Publication

Bui et al Nat Commun. 2025 Apr 26;16(1):3932. doi:

Insert

SNAPtag-PTS1

Use

Lentiviral

Tags

SNAPtag

Expression

Mammalian

Mutation

no

Promoter

CMV

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Publication

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Expression

Yeast

Promoter

tetO7 promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-DNMT3a-DNMT3l

Plasmid

#154140

Purpose

Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

Depositor

Albert Jeltsch

Publication

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP

Tags

HA and sfGFP

Expression

Mammalian

Promoter

SFFV

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Triple ORF LoxP ifgMosaic Donor (LH208)

Plasmid

#99625

Purpose

To clone the 3 ORFs for Cre dependent generation of ifgMosaics

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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