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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

Plasmid

#47542

Purpose

engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

Depositor

Henry Lester

Article

Frazier et al J Biol Chem. 2013 May 29.

Insert

Glutamate-gated chloride channels

Expression

Mammalian

Mutation

retention motif in ER (starting at R340) changed …

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127321

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 DLC1

Plasmid

#24266

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1, DLC1 Human)

Tags

T7

Expression

Mammalian

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
hTRAFD1-pCMV14-3xFlag

Plasmid

#73587

Purpose

Express human TRAFD1 (FLN29) in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

The original vector hTRAFD1-mCMV14-Flag was a gif…

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTYF-PRSx8-AstR-p2A-GFP

Plasmid

#159632

Purpose

Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

Depositor

Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

AstR (AstA-R1 Fly)

Use

Lentiviral

Tags

GFP and P2A

Expression

Mammalian

Promoter

PRSx8

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-CERT-PH

Plasmid

#49572

Purpose

expresses the plekstrin homology domain of CERT/OSBP in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

Insert

GPBP (CERT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

expresses AA 1-122

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
Erk2

Plasmid

#40777

Purpose

Expression of rat Erk2 in mammalian cells

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

Erk2 (Mapk1 Rattus norvegicus)

Tags

eGFP

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-RBP-GFP(S45C)-ZdK1

Plasmid

#185791

Purpose

Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

Depositor

Lizhong He

Article

Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

Insert

RBP-sfGFP(S45C)-ZdK1

Tags

10xHis, TEV protease cleavage site after His Tag

Expression

Bacterial

Mutation

Ser to Cys mutation at start of GFP, residue 45 i…

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) pCMV

Plasmid

#8539

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pDEST15.1-hRPN13 (285-407)

Plasmid

#61934

Purpose

Expression of hRPN13 (285-407) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-407) (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
SRAcode_in_pUC57_v3

Plasmid

#49822

Purpose

for transcribing SRA nucl 419-1356 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3(767-1356)

Plasmid

#49826

Purpose

for transcribing SRA nucl 767-1356 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-CERT PH domain

Plasmid

#58725

Purpose

Expresses GFP-Tagged CERT PH domain in yeast

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
HOXC4 (human) MSCV

Plasmid

#8546

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXC4 (HOXC4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains improved ribosomal binding site and NcoI…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBT259_(pRosa26-G-tTA2)

Plasmid

#36878

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
diphteria toxin A

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron(Neo)-T(FLLFLNL)

Plasmid

#40020

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdT-3Myc
beta-globin intron
Neo

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBT250_(pCA-G-intron(Neo)-tTA2)

Plasmid

#36876

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hmga2 ATG wt

Plasmid

#52034

Purpose

encodes wt HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

starting ATG deleted

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only