We narrowed to 6,106 results for: cat.2

1. pk335-CARGO-12mer-14kb-USF-1-12

   Plasmid

   #227472

   Purpose

   12-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 14kb Upstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-ERK2

   Plasmid

   #118296

   Purpose

   Expression of Rat ERK2

   Depositor

   Philip Stork

   Article

   Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR4 CDS A. thaliana PARP2

   Plasmid

   #175810

   Purpose

   pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   PARP2 (PARP2 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Expression

   Mutation

   Promoter

   none

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKERep

   Plasmid

   #211826

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKESa

   Plasmid

   #211828

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKERepPar

   Plasmid

   #211827

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-ADARcd-E488Q-V5

   Plasmid

   #139686

   Purpose

   Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Adenosine deaminase acting on RNA (Adar Fly)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

   Plasmid

   #196973

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag

   Depositor

   Lukas Kapitein

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   GFP and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

   Plasmid

   #196976

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST

   Depositor

   Lukas Kapitein

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   GST and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBP-J23100

    Plasmid

    #72963

    Purpose

    Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23100 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBP-pET-RBS

    Plasmid

    #72981

    Purpose

    PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    pET RBS derived from pET vectors
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FH95pUP95PDZGW (C6)

    Plasmid

    #74022

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    PDZ

    Promoter

    H1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

13. FH95pUP95Q15AD45_64GW (D8)

    Plasmid

    #74031

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-Q15A _45_64-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Q15A, D45_64

    Promoter

    H1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

14. FH95pUP95L460pGW (E2)

    Plasmid

    #74032

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-L460P-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    L460P

    Promoter

    H1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

15. FH95pUP95DSKIrGW (D2)

    Plasmid

    #74025

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95dSK and EGFP

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    delta SK

    Promoter

    H1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pBP-J23108

    Plasmid

    #72964

    Purpose

    Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23108 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBP-J23114

    Plasmid

    #72965

    Purpose

    Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23114 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBP-T7_RBS

    Plasmid

    #72978

    Purpose

    T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7+RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBP-Strep(II)

    Plasmid

    #72984

    Purpose

    Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Strep(II) N-terminal tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBP-lacZ

    Plasmid

    #72948

    Purpose

    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - Bioparts
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge