We narrowed to 5,820 results for: pcas

1. pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

   Plasmid

   #232246

   Purpose

   Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   delta73-82

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA528 IST1 L328D,L355A (316-366)(C-Cys)

   Plasmid

   #193037

   Purpose

   Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D and L355A mutations that diminishes binding to MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L328D, L355A, Residues 316-366, Non-native C-term…

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pzk228-pmax-SgrAI[3xFLAG]-AscI-dPspCas13b(AAAA)

   Plasmid

   #196827

   Purpose

   Express dpdpCas13b with AAAA mutation

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   dpspCas13b(AAAA)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   K367A, D369A and K370A

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

   Plasmid

   #211815

   Purpose

   lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   SpCas9, tracrRNA and puromycin selection gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U1a promoter, U6 promoter, and hPGK promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-GST-ATG13 (460-517)-L476/L479D

   Plasmid

   #210845

   Purpose

   Mammalian expression of ATG13 (460-517) mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Expression

   Mammalian

   Mutation

   L476/L479D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-GST-ATG13 (450-517)-A452D/F453D

   Plasmid

   #210851

   Purpose

   Mammalian expression of ATG13 (450-517) mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Expression

   Mammalian

   Mutation

   A452D/F453D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-GST-ATG13 (460-517)-F509/F512D

   Plasmid

   #210847

   Purpose

   Mammalian expression of ATG13 (460-517) mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Expression

   Mammalian

   Mutation

   F509/F512D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

   Plasmid

   #210842

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Expression

   Mammalian

   Mutation

   L324D/R543D/R548D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-GST-ATG13 (460-517)-L495/L499D

   Plasmid

   #210846

   Purpose

   Mammalian expression of ATG13 (460-517) mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Expression

   Mammalian

   Mutation

   L495/L499D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-GST-ATG13 (460-517)-F466D/F470D

    Plasmid

    #210844

    Purpose

    Mammalian expression of ATG13 (460-517) mutant

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Expression

    Mammalian

    Mutation

    F466D/F470D

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

    Plasmid

    #188272

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    AaCas12b(Q119F/E475R/E758R)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

    Plasmid

    #188274

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    PlmCasX(T26R/K610R/K808R)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

    Plasmid

    #196877

    Purpose

    Neuron-specific expression of mOrange2 reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

    Plasmid

    #196874

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynGFP
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

    Plasmid

    #196884

    Purpose

    Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-Lyn-GFP-bGH
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

    Plasmid

    #199451

    Purpose

    CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-GST-ATG9 (828-839)-ATG101 (1-198)

    Plasmid

    #188073

    Purpose

    To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 and ATG101 (ATG9A, ATG101 Human)
    

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    codon-optimized ATG9 (828-839) and codon-optimize…

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

    Plasmid

    #179007

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

    Plasmid

    #179006

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

    Plasmid

    #179001

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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