We narrowed to 7,267 results for: trac

1. pTT3 Unc5B-FLAG

   Plasmid

   #72195

   Purpose

   Expresses full-length Unc5b with a C-terminal FLAG tag

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5b (Unc5b Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. cyt-GafD-mTurboID-V5

   Plasmid

   #184640

   Purpose

   Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   cyt-GlycoID-V5
   

   Use

   Tags

   Nuclear export sequence and V5 tag

   Expression

   Mammalian

   Mutation

   GafD -> expressed aa22-178 ; BirA* -> expre…

   Promoter

   CMV

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2155

   Plasmid

   #141065

   Purpose

   AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

   Insert

   SYN1-EmGFP-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   April 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. eGFP-KIF1A-Rigor

   Plasmid

   #172207

   Purpose

   Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

   Insert

   KIF1A (Kif1a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 251 to Alanine

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174644

   Purpose

   Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Venus-SSPB and iLID-mCherry

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. nuc-GafD-mTurboID-HA

   Plasmid

   #184641

   Purpose

   Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   nuc-GlycoID-HA
   

   Use

   Tags

   3xHA tag and Nuclear localization sequence

   Expression

   Mammalian

   Mutation

   GafD -> expressed 22-178 ; BirA* -> express…

   Promoter

   CMV

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST-HisMBP-SED1

   Plasmid

   #178191

   Purpose

   SED1 biosensor for expression in Escherichia coli

   Depositor

   Jose Dinneny, Cesar Cuevas-Velazquez

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 biosensor
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p51.SRE.mCMV.CD63-GFP.wpre

   Plasmid

   #226773

   Purpose

   CD63-GFP in p52 (SRE backbone)

   Depositor

   Ferdinando Pucci

   Article

   Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

   Insert

   CD63 (CD63 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB80-LAMP1-mCherry-iLID

   Plasmid

   #174625

   Purpose

   Optogenetic coupling to lysosomes (LAMP1) via iLID

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   LAMP1-mCherry-iLID (LAMP1 Human, Synthetic)
   

   Use

   Tags

   mCherry-iLID

   Expression

   Mammalian

   Mutation

   mCherry: Met1Del

   Promoter

   Chicken beta-actin

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEMS-Halo7Tag-hFis

    Plasmid

    #111136

    Purpose

    hFis with selflabeling tag, e.g. for fluorescence microscopy

    Depositor

    Karin Busch

    Article

    Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

    Insert

    human fission factor1 (FIS1 Human)
    

    Use

    Tags

    Halo7-tag

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-hSyn-Axon-jGCaMP8s

    Plasmid

    #172720

    Purpose

    AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Axon-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTT3 Unc5C-FLAG

    Plasmid

    #72196

    Purpose

    Expresses full-length Unc5c with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c (Unc5c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-hPPP3CC-FLAG

    Plasmid

    #179162

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEMS-Tom20-Halo7Tag

    Plasmid

    #111135

    Purpose

    Tom20 with selflabeling tag, e.g. for fluorescence microscopy

    Depositor

    Karin Busch

    Article

    Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

    Insert

    Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
    

    Use

    Tags

    Halo7 Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-TRE-NS1NF

    Plasmid

    #175279

    Purpose

    AAV vector mediating inducible expression of the NS1 gene of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    NS1 (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tight TRE

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. loxP-myctagBFP-STOP-loxP-mCherry-NTR

    Plasmid

    #108456

    Purpose

    This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

    Insert

    loxP-myctagBFP-STOP-loxP-mCherry-NTR
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-DIO-NS1

    Plasmid

    #175273

    Purpose

    AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HaloTag-WDR5

    Plasmid

    #200880

    Purpose

    Mammalian expression of human WDR5 with N-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    HaloTag-WDR5 (WDR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-mRuby3-MLC-IRES-Neo

    Plasmid

    #222635

    Purpose

    Reporter for activated NM2

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mRuby3-MLC (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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