Addgene

We narrowed to 825 results for: 3xha

Showing: 261 - 280 of 825 results

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PX552-mScn8a-3xHA-Syn-smFP-flag

Plasmid

#182563

Purpose

For mouse Nav1.6 knockin with 3xHA at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xHA donor DNA
smFP-flag

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EF1 and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107321

Purpose

Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to SaCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

Plasmid

#69221

Purpose

Expresses R1333K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Lysine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

Plasmid

#69222

Purpose

Expresses R1333S mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Serine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

Plasmid

#209293

Purpose

mitoBE expression vector, target mtND4 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

Plasmid

#209294

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

SOD2-3xHA-TALE(RNR2-Left)-MutH

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

Plasmid

#170327

Purpose

Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107320

Purpose

Expresses SpCas9 fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to SaCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

Plasmid

#170317

Purpose

Expresses HA and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

Plasmid

#178960

Purpose

Expresses saCas9 protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Staphylococcus aureus Cas9 (SaCas9)

Use

AAV

Tags

3x HA

Expression

Mutation

Promoter

gfaABC1D (astrocyte specific)

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo

Plasmid

#170319

Purpose

Expresses HA and EGFP tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

Plasmid

#170321

Purpose

Expresses HA and mCherry tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)

Plasmid

#73477

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-3XHA-P2A-N2c(G)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

Plasmid

#32703

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

3x HA tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

Plasmid

#214998

Purpose

Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)

Use

Hdr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

Plasmid

#214999

Purpose

Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)

Use

Hdr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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DIO-EF1a-FLEX-H2B-3XHA-P2A-N2c(G)

Plasmid

#73478

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-3XHA-P2A-N2c(G)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

Plasmid

#104563

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1 (D832A)

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

Plasmid

#153008

Purpose

Expresses glucagon peptide on mammalian cell surface

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

Glucagon-ICAM1-pre-mGRASP

Use

Tags

3x HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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