Addgene

We narrowed to 1,167 results for: Np

Showing: 261 - 280 of 1167 results

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pJPT028-40 Angstrom 12TMD de novo_mRFP1_3x FLAG

Plasmid

#202510

Purpose

Express 40 Angstrom 12TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

40 Angstrom 12TMD de novo

Use

Tags

mRFP1-3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT024-24 Angstrom 4TMD de novo_mRFP1_3x FLAG

Plasmid

#202506

Purpose

Express 24 Angstrom 4TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

24 Angstrom 4TMD de novo

Use

Tags

mRFP1-3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT027-40 Angstrom 4TMD de novo_mRFP1_3x FLAG

Plasmid

#202509

Purpose

Express 40 Angstrom 4TMD de novo protein

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

40 Angstrom 4TMD de novo

Use

Tags

mRFP1-3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT009-ZF6 PM-synTF-1x FLAG

Plasmid

#202499

Purpose

Express "PM" synTF (ZF6)

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

p65-ZF6

Use

Tags

1x FLAG and Palmitoylation-Myristoylation Site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-AcrA

Plasmid

#198214

Purpose

pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

AcrA

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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1056H

Plasmid

#183135

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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1056J

Plasmid

#183136

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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1056K

Plasmid

#183137

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

Plasmid

#149427

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR[dsxM]

Plasmid

#131616

Purpose

Expresses a functional PuroR only in males and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM] (dsx Fly)
dsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2 and hr5Ie1

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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gRNA[Tra].1026G

Plasmid

#112689

Purpose

express gRNA targeting Tra under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[Tra] (tra Fly)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Use

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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MSGV1-1D3-28Z.1-3 mut

Plasmid

#107227

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs mutated

Depositor

Steven Rosenberg, James Kochenderfer

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z. 1-3 mut

Use

Retroviral

Tags

None

Expression

Mutation

None

Promoter

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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MSGV-1D3-28Z All ITAMs intact

Plasmid

#107226

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs intact

Depositor

Steven Rosenberg, James Kochenderfer

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z All ITAMs intact

Use

Retroviral

Tags

None

Expression

Mutation

None

Promoter

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pPRIME-dsRed-shscramble

Plasmid

#71384

Purpose

Expresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cells

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

CMV promoter-dsRed-mir30-shscramble

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer/promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF-1b-RFC140ΔN555

Plasmid

#175043

Purpose

Overexpression of human (Rfc1) p140ΔN555 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 1 (RFC1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCherry-mNrp1

Plasmid

#21934

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Tags

Cherry and His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only
CapChR1_pmCherry-N1

Plasmid

#188031

Purpose

Expresses CapChR1-mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR1-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

S63D, L132C, T159C and N258E mutations in CrChR2

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster nanos

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB1-eGFP-6xHis

Plasmid

#130981

Purpose

Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-GFP-6xHis (Mapre1 Mouse)

Use

Tags

eGFP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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