We narrowed to 22,648 results for: CRISPR

1. pDM068

   Plasmid

   #216811

   Purpose

   Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Type

   Empty backbone

   Use

   CRISPR; Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRhaCAST

   Plasmid

   #211791

   Purpose

   pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter

   Depositor

   Silvia Cardona

   Article

   Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Rhamnose-inducible

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF823_EFS-Cas9-P2A-Puro

   Plasmid

   #211645

   Purpose

   EFS-Cas9-P2A-Puro

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF226_EFS-Cas9-P2A-Puro

   Plasmid

   #211644

   Purpose

   EFS-Cas9-P2A-Puro

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL28_Cas9-His Δcys E532C/E1207C

   Plasmid

   #206289

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E1207C

   Promoter

   T7

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDL27_Cas9-His Δcys E532C/E945C

   Plasmid

   #206288

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E945C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL26_Cas9-His Δcys M1C/E532C

   Plasmid

   #206287

   Purpose

   Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, E532C, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Cas12i2-T2A-EGFP

   Plasmid

   #188270

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-AaCas12b-T2A-EGFP

   Plasmid

   #188271

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   AaCas12b
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BPK2014-Cas12i-Max

    Plasmid

    #188276

    Purpose

    prokaryotic expression

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i-Max
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lenti-Cas9-sgHPRT1

    Plasmid

    #196713

    Purpose

    CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Insert

    Cas9-T2A-BSD-U6-sgHPRT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a/hU6

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcrVIB1-cloDF-amp

    Plasmid

    #184843

    Purpose

    Expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPgiCas13b

    Plasmid

    #184833

    Purpose

    Inducible expression of PgiCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Cas13b nuclease from Porphyromonas gingivalis
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. DpbdCas12e VPR

    Plasmid

    #188515

    Purpose

    Expresses FLAG tagged DpbdCas12e VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D672A/E769A/D935A

    Promoter

    EF1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. DpbCas12e

    Plasmid

    #188495

    Purpose

    Expresses FLAG tagged DpbCas12e

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PlmCas12e

    Plasmid

    #188496

    Purpose

    Expresses FLAG tagged PlmCas12e

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas12j3

    Plasmid

    #188497

    Purpose

    Expresses FLAG tagged Cas12j3

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

    Plasmid

    #167899

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

    Plasmid

    #167897

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

20. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

    Plasmid

    #167903

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only