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pBS-KS-attB1-2-PT-SA-SD-1-GFP11

Plasmid

#172068

Purpose

Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-4T-2/hGGA3(GAT)

Plasmid

#79436

Purpose

Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA3 (GGA3 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 107-286

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFX-mito-eqFP650

Plasmid

#174179

Purpose

Express eqFP650 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

eqFP650 with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mKate2-Rab11

Plasmid

#174463

Purpose

Express ｍKate2 with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mKate2 with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1 Aquamarine

Plasmid

#106285

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Aquamarine

Tags

6xHis and TEV cleavage (N terminal on insert)

Expression

Bacterial

Mutation

T65S and H148G

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Sec61β

Plasmid

#174466

Purpose

Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Tags

Sec61 subunit beta

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET30a-FluA-NP

Plasmid

#165092

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFX-mito-roGFP

Plasmid

#174181

Purpose

Express roGFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

roGFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG MSCV

Plasmid

#8515

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Use

Retroviral

Tags

FLAG (DYKDDDDK) and IRES-GFP

Expression

Mammalian

Mutation

makes GFP from an IRES driving translation from a…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pAB092a

Plasmid

#79217

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAB078d8

Plasmid

#79206

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET17b-yebST

Plasmid

#175804

Purpose

Complementation of yebST knockout in E. coli K-12

Depositor

Gira Bhabha, Damian Ekiert, Ian Henderson

Article

Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

Insert

yebST

Tags

None

Expression

Bacterial

Mutation

silent mutations to introduce restriction sites

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-Venus

Plasmid

#175840

Purpose

Express Venus with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with nucleus-targeting sequence

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA4

Plasmid

#136662

Purpose

Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA4

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas13d-T2A-GFP(1-10)

Plasmid

#224566

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-GFP(1-10)

Use

CRISPR

Tags

T2A-split GFP(1-10)

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-iRFP

Plasmid

#175842

Purpose

Express iRFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with nucleus-targeting sequence

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAB078d3

Plasmid

#79203

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p6614 MSCV-IP N-HAonly PXMP3

Plasmid

#35012

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PXMP3 (PEX2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-Cas13d-T2A-Citrine

Plasmid

#224565

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-Citrine

Use

CRISPR

Tags

T2A-Citrine

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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