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Sema3e(L)-Fc-His

Plasmid

#72145

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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p164 Dync1i1.B

Plasmid

#26435

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pCas4_1_2_VA

Plasmid

#186438

Purpose

Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIG-Flag-Sox9-IRES-nls-GFP

Plasmid

#44281

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

Flag-Sox9 (SOX9 Chicken)

Tags

Flag

Expression

Mammalian

Promoter

CMV-IE enhancer and Chicken beta-actin promoter

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K13R

Plasmid

#63566

Purpose

Expression of human H2A with K13R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 13 is now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K15R

Plasmid

#63567

Purpose

Expression of human H2A with K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 15 is now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
ATP1A1_G4_Q118R_Dual_pegRNA

Plasmid

#173199

Purpose

Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G4 Q118R pegRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1(delta CTD)

Plasmid

#1030

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PGC1a (Ppargc1a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

delta CTD

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pHH0103_EIF4E_IF4E

Plasmid

#110010

Purpose

Protein expression and purification of EIF4E_IF4E

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EIF4E_IF4E (EIF4E Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-mHopX

Plasmid

#16425

Depositor

Jonathan Epstein

Article

Chen et al Cell. 2002 Sep 20. 110(6):713-23.

Insert

Hopx (hop Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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HOXB2 (human) HIS-tag pET

Plasmid

#8522

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB2 (HOXB2 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Starts at AA #2; makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCS2-ER-EmEGFP

Plasmid

#169000

Purpose

Express ER-EmEGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)

Use

Unspecified

Tags

EGFP

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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Sema6d.1-Fc-His

Plasmid

#72168

Purpose

Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.1 (Sema6d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-NLS-mut

Plasmid

#42000

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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MigR1 Fli-1 GFP

Plasmid

#24668

Depositor

Yaacov Ben-David

Article

Cui et al Blood. 2007 Oct 1. 110(7):2631-40.

Insert

Fli-1 (Fli1 Mouse)

Use

Retroviral

Tags

GFP and IRES

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
Sema3f(L,+)-AP-His

Plasmid

#72021

Purpose

Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.1-AP-His

Plasmid

#72042

Purpose

Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.1 (Sema6d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAT3_G-alpha

Plasmid

#109948

Purpose

Protein expression and purification of GNAT3_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAT3_G-alpha (GNAT3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL2-R244Q

Plasmid

#109023

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-R244Q (ATL2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Arg244Gln mutation results in dimerization defect

Promoter

EIF-1a promoter

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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