We narrowed to 12,189 results for: LEA
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Plasmid#59762PurposeExpress Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRCC1
ExpressionMammalianPromoterCMV and T7Available sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6
Plasmid#20277DepositorAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-P10HA
Plasmid#1345DepositorAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)
Plasmid#169160PurposeTo express nsp14-nsp10 fusion protein in E. coliDepositorInsert14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
Tags14His-SUMO and Fusion protein of SARS-CoV-2 nsp14ā¦ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iSeroSnFR-Nlgn (AAV9)
Viral prep#128485-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-iSeroSnFR-Nlgn (#128485). In addition to the viral particles, you will also receive purified pAAV-CAG-iSeroSnFR-Nlgn plasmid DNA. CAG-driven expression of a fluorescent reporter for serotonin (localized to dendrites). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-FLAG
Plasmid#119754PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.DepositorTypeEmpty backboneTagsFLAG and GSTExpressionBacterialPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe(SV40)-Cytochrome C-GFP
Plasmid#41184DepositorInsertCytochrome C-GFP
UseRetroviralExpressionMammalianPromoterSV40Available sinceNov. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEMS1052
Plasmid#29004PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable sinceAug. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear locaā¦ExpressionMammalianMutationSee depositor comments belowAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE
Plasmid#89587PurposeFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.DepositorInsertnuclear localization signal (nls) turboRFP
ExpressionMammalianAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA-gp78-FLAG RING mutant C356G H361A
Plasmid#61751Purposeexpress C-terminally FLAG tagged human gp78DepositorInsertgp78 (AMFR Human)
TagsFLAGExpressionMammalianMutationC356G H361A; P179L, F262L, Y315C, and D346G (pleā¦PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro_shDDX58_230212
Plasmid#167293PurposeLentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.DepositorAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-NLGN3 R451C
Plasmid#59319PurposeExpression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutationDepositorInsertNLGN3 R451C (NLGN3 Human)
TagsHAExpressionMammalianMutationArginine 451 to Cysteine (R451C mutation)PromoterCAGAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS1172
Plasmid#29301PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ220-Ultra
Plasmid#176889PurposeGateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoterDepositorInsertAsCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
Tags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY006 (pcDNA3.1-hBpCpf1)
Plasmid#69978PurposeExpresses humanized BpCpf1DepositorInserthBpCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PET-HiFi SpCas9-NLS-6xHis
Plasmid#207376PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OT1.0
Plasmid#185387PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin sensor GRAB_OT1.0
UseAAVPromoterEFSAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_Halo_GluA2
Plasmid#170454PurposeExpresses halo tagged GluA2 in neuronal cellsDepositorPublicationAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by