We narrowed to 150,487 results for: ins

1. pFX-β-tubulin-Venus

   Plasmid

   #175828

   Purpose

   Express Venus with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with microtuble-targeting sequence
   

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFX-Lifeact-mCherry

   Plasmid

   #175822

   Purpose

   Express mCherry with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with actin-targeting sequence
   

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Promoter

   CMB

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CapChR2_pUAST-attb

   Plasmid

   #188041

   Purpose

   Expresses CapChR2 in Drosophila

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2
   

   Tags

   none

   Expression

   Insect

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   UAS

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. S12.1_Empty_Bridge

   Plasmid

   #127670

   Purpose

   Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-mito-MiCy

   Plasmid

   #174177

   Purpose

   Express MiCy with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   MiCy with mitochondria-targeting sequence
   

   Tags

   the mitochondria-targeting sequence of the cytoch…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p6604 MSCV-IP N-HAonly PP2CA

   Plasmid

   #35005

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   PP2CA (PP2CA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pBXNH3LCA_TM287/288

   Plasmid

   #132701

   Purpose

   expresses TM287/288 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   TM287/288 (TM0288, TM0287 Thermotoga maritima)
   

   Tags

   Avi and His

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB088c

   Plasmid

   #79213

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFX-β-tubulin-iRFP

   Plasmid

   #175830

   Purpose

   Express iRFP with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with microtuble-targeting sequence
   

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-nef-CIAO-TVA-mCherry

    Plasmid

    #149295

    Purpose

    Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    TVA:mCherry
    

    Use

    AAV

    Mutation

    Start codon removed.

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. S12.2_Bridge + Target

    Plasmid

    #127671

    Purpose

    Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-Venus-Sec61β

    Plasmid

    #174467

    Purpose

    Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with endoplasmic reticulum-targeting sequence
    

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-30a(+)-Syk-tSH2

    Plasmid

    #111269

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS DKK3

    Plasmid

    #19201

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Dickkopf homolog 3 (DKK3 Chicken)
    

    Expression

    Bacterial

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pFX-H2B-EGFP

    Plasmid

    #175839

    Purpose

    Express EGFP with nucleus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with nucleus-targeting sequence
    

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2XMyc-LRRK2-deltaHeat

    Plasmid

    #25068

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains the LRR-ROC-COR-Kinase-WD40 domains; Del…

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pFX-β-tubulin-SECFP

    Plasmid

    #175826

    Purpose

    Express SECFP with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with microtuble-targeting sequence
    

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFX-EGFP-KRasCT

    Plasmid

    #175833

    Purpose

    Express EGFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFX-mCherry-Sec61β

    Plasmid

    #174468

    Purpose

    Express mCherry with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with endoplasmic reticulum-targeting sequence
    

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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