We narrowed to 34,882 results for: CaS;

1. pRS315e_pGal-nCas9(D10A)-PmCDA1

   Plasmid

   #79617

   Purpose

   Expresses nCas9(D10A)-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CuO-V5 CASC3 siRes 1-480 WT

   Plasmid

   #158541

   Purpose

   For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480

   Depositor

   Niels Gehring

   Article

   Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

   Insert

   CASC3; BTZ; MLN51 (CASC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Amino acids 1-480, C-terminal deletion

   Promoter

   CMV

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_mouse-Caspase-11_enzymatic-domain

   Plasmid

   #183382

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-11 (AA100-373) (Casp4 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC2-dual-dCas9VP48-sgExpression

   Plasmid

   #48236

   Purpose

   Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9VP48
   

   Use

   CRISPR

   Tags

   HA-tag and VP48

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH-Ubi-miniSdd7-nCas9-PBE

   Plasmid

   #204856

   Purpose

   For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd7-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   ZmUbi-1, OsU3, 35S

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-1BPNLS-Cas9-1BPNLS

   Plasmid

   #87108

   Purpose

   BPNLS-Cas9-BPNLS expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/pMXs-neo

   Plasmid

   #51260

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174840

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ENO1p

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5 CASTOR1-Flag Cterm

   Plasmid

   #84488

   Purpose

   overexpression of CASTOR1 with Flag tag

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 (GATSL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pAAVS1-CAG-hCAS9-neo

    Plasmid

    #166026

    Purpose

    AAVS1 targeting vector carrying CAG-SpCas9-neo

    Depositor

    Kosuke Yusa

    Insert

    AAVS1-CAGCas9IRESneo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-AsCas12f1-v5.1+T1-empty

    Plasmid

    #204977

    Purpose

    AsCas12f1-v5.1+T1-based Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nature Catalysis (2023) 6, 695–709

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N70Q/K103R/A104R/S118A/D364R

    Promoter

    PCMV,U6

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZ148-BhCas12b-sgRNA-scaffold

    Plasmid

    #122448

    Purpose

    Expresses sgRNA for BhCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BhCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ607-CMV-GFP-2A-Cas9

    Plasmid

    #154830

    Purpose

    GFP-2A-Cas9 (CHO codon-optimized)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    GFP-2A-Cas9
    HygR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized (Cricetulus griseus)

    Promoter

    CMV and SV40 promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SMVP-Cas9N-U6-gRNA P1

    Plasmid

    #80943

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK 3xFLAG-BCAS2 zeo

    Plasmid

    #192319

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-BCAS2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BCAS2 (BCAS2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. xCas9(3.6)-ABE(7.10)

    Plasmid

    #108387

    Purpose

    Mammalian expression vector for xCas9(3.6)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-ABE(7.10)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. R26-dCas9-VPR-mNG

    Plasmid

    #172441

    Purpose

    Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    dCas9-VPR
    mNeonGreen
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK-caCas9-NatMX-Neut5L

    Plasmid

    #89576

    Purpose

    caCas9 integrating vector into the Neut5L locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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