We narrowed to 28,008 results for: STI;
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Plasmid#109329PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selectionDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only
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Sox1/2/3-2373/+3>nls::Cas9::nls
Plasmid#59989PurposeSox1/2/3 (SoxB1) driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Sox1/2/3 (SoxB1) -2373/+3Available SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferasePromotersgkAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK2301
Plasmid#65778PurposeHuman expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNADepositorInsertSt1Cas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-NfsB.zf1
Plasmid#61387PurposemRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafishDepositorInsertnfsB.zf1
UseZebrafish expressionMutationE28GPromotersp6Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NGFP-mAID
Plasmid#140607PurposeExpression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1
ExpressionMammalianAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-sfGFP
Plasmid#213863PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused sfGFP
ExpressionBacterial and PlantAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianPromoterCAGAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PM
Plasmid#197386PurposeTo constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).DepositorInsertPDX1-T2A-MAFA
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKERep
Plasmid#211826PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESa
Plasmid#211828PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NmCherry-mAID
Plasmid#140603PurposeExpression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1
ExpressionMammalianAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NmCherry-mAID
Plasmid#140601PurposeExpression of OsTIR1 and mCherry-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
ExpressionMammalianAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0620
Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECFP-Cdc42Q61L
Plasmid#105294PurposeConstitutively active Cdc42DepositorAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0750
Plasmid#115511PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.DepositorInsertgusA pOGG083, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-mKdm4d(H189A)-polyA
Plasmid#61554PurposeFor mRNA synthesis or expression in mammalian cellsDepositorInsertKdm4d (catalytic mutant)
TagsflagExpressionMammalianMutationchanged Histidine 189 (of Kdm4d) to AlaninePromoterCMV and T7Available SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0751
Plasmid#133485PurposeExpression in fission yeast from promoter pnmt1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only