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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 2821 - 2840 of 2854 results

  1. pJK215

    Plasmid
    #72427
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Inserts
    succinyl-diaminopimelate desuccinylase
    2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
    acetylglutamate kinase
    ribosome biogenesis GTP-binding protein YsxC
    insertase
    ribonuclease P
    50S ribosomal protein L34
    hypothetical protein
    glyoxylase I
    hypothetical protein
    UDP-N-acetylglucosamine acyltransferase
    3-hydroxyacyl-ACP dehydratase
    UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Mutation
    missing C-terminal domain residues 277-391(ter) a…
    Promoter
    n/a
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72427-plasmid-map-sequence-id-137744

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  2. pLentiCMV_HyPer7RgDAAO

    Plasmid
    #217653
    Purpose
    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Use
    Lentiviral
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    CMV
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217653-plasmid-map-sequence-id-441459

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  6. pTriEx-PA-Rac1-C450A

    Plasmid
    #22025
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains light-insensitive mutation C4…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  7. pTriEx-PA-Rac1-I539E

    Plasmid
    #22026
    Purpose
    Expression of photoactivatable Rac1 ('lit' state mutant)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains 'lit state' mutatio…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  9. pTriEx-mVenus-PA-Rac1-C450A

    Plasmid
    #22021
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains the light-insensitive C450A m…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  10. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

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  12. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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  16. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid
    #198914
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    exon 18a deleted, exon 19 starts 3 nucleotides ea…
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198914-plasmid-map-sequence-id-392117

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Showing: 2821 - 2840 of 2854 results