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R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

Plasmid

#159575

Purpose

Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)

Tags

His6-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-β2AR-μDpTT

Plasmid

#182326

Purpose

Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

beta2AR-μDronpa-TagRFP-T (ADRB2 Human)

Tags

Full-length human beta2 adrenergic receptor

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmarca4#1/Cre

Plasmid

#173619

Purpose

Expresses a Smarca4-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Smarca4 (Smarca4 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-SRSF1 siRNA-resitant

Plasmid

#218974

Purpose

Vector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

SRSF1 cDNA

Expression

Mammalian

Mutation

siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…

Promoter

CMV

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-enCas12a-2xNLS

Plasmid

#209021

Purpose

Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

enCas12a-2xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

E174R/S542R/K548R

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB-Hygro

Plasmid

#176056

Purpose

EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2_LacZ_sgRNA_2

Plasmid

#155093

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CaMK2rep-S603A

Plasmid

#239623

Purpose

Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

Depositor

F Javier Díez Guerra

Article

Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

Insert

SpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)

Tags

Spot, myc, and sGFP2

Expression

Mammalian

Mutation

changed Serine 603 to Alanine

Promoter

CMV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

Plasmid

#166010

Purpose

mammalian expression of SARS-CoV-2 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTNwt-FL

Plasmid

#110217

Purpose

Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Expression

Bacterial

Mutation

P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc Affimer6-msfGFPΔN12

Plasmid

#231550

Purpose

Mammalian expression of actin-binding Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Tags

msfGFP

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA

Plasmid

#174900

Purpose

amber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Front Chem. 2021 Nov 11;9:768535. doi: 1

Insert

CRFR1 (Crhr1 Rat)

Tags

HA

Expression

Mammalian

Mutation

A95TAG

Promoter

EF1

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAF92

Plasmid

#135929

Purpose

All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Insert

mCherry fused to TNFSF9

Use

Transposon

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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plentiCRISPv2_FDX1-3

Plasmid

#184490

Purpose

Lentivirus all in one CRISPR vector targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCRISPv2_FDX1-1

Plasmid

#184488

Purpose

Lentivirus all in one CRISPR vector targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCRISPv2_FDX1-2

Plasmid

#184489

Purpose

Lentivirus all in one CRISPR vector targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETV1

Plasmid

#98623

Purpose

Gateway entry TOPO TA cloning vector for ETV1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETV1 (Etv1 Mouse)

Use

Topo ta cloning vector

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_WT_ADRB2_LucReporter

Plasmid

#129788

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Promoter

N/A

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmarca4#2/Cre

Plasmid

#173620

Purpose

Expresses a Smarca4-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Smarca4 (Smarca4 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(WT)

Plasmid

#177131

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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