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pGEMHE:mTREK-1(K271Q)

Plasmid

#133270

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )

Use

Oocyte expression

Mutation

K271Q

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#133077

Purpose

X. laevis expression vector. It will generate the human TRAAK channel

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)

Mutation

Q258K

Promoter

T7

Available Since

Oct. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_1

Plasmid

#106306

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pY71-PT7-RiboJ-sfGFP-MGapt

Plasmid

#129119

Purpose

Expresses a fluorescent reporter for the quantification of cell-free protein expression.

Depositor

Matthew Lux

Article

Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

Insert

Superfolder GFP with Malachite Green aptamer

Use

Synthetic Biology

Tags

His6 and RiboJ Insulator

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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XE157 GFP-XDpr-delta PDZB-CS2+

Plasmid

#16765

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-delta PDZB (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Mutation

XDpr is truncated to delete the last four amino a…

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pNIA-CEN-Venus-FLAG-LINXa4-Bre1

Plasmid

#73618

Purpose

Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

Venus

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A

Promoter

ADH1

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Cacnb1 KI

Plasmid

#139660

Purpose

Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES- mCE(K294A)

Plasmid

#82464

Purpose

Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Mutation

K294A

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-PM BRD7 sh1

Plasmid

#41927

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pPICZ:hTRAAK(G124I)

Plasmid

#130672

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, G124I

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgFTCD_2

Plasmid

#102313

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_2 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP010

Plasmid

#101166

Purpose

E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Insert

HH-gRNA-HDV targetting SeILV6 in S. pastorianus

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-PM BRD7 sh3

Plasmid

#41930

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
CtkA_full_cmv-10

Plasmid

#27433

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

3xFLAG

Expression

Mammalian

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q/D179Q_cmv-10

Plasmid

#27435

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

3xFLAG

Expression

Mammalian

Mutation

D155Q/D179Q

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_full_pET21a

Plasmid

#27428

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q_pET21a

Plasmid

#27430

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D155Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D179Q_pET21a

Plasmid

#27431

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D179Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q/D179Q_pET21a

Plasmid

#27432

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D155Q/D179Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
pX459-Fzd7 gRNA

Plasmid

#246566

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd7 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd7 gRNA (Fzd7 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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