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Plasmid#106379PurposeCan be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulationDepositorInsertGlmS with M9 mutation
UseTagsExpressionMutationPromoterAvailable sinceMarch 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
3TERM_Nos
Plasmid#136115Purpose3TERM after CDS/ CDS12-CTAGDepositorInsert3TERM_Nos (A. tumefaciens)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMaCTag-11
Plasmid#119990PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-iDepositorInsertmScarlet-i
UsePcr templateTagsmScarlet-iExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PURO-PTP
Plasmid#51711Purposecontains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)ExpressionMutationPromoterAvailable sinceMarch 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Sec23A
Plasmid#66611PurposeYFP fusion of Sec23ADepositorInsertSec23A (SEC23A Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP
Plasmid#84256PurposeExpresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent markerDepositorInsertSa sgCXCR4-3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEcCas6
Plasmid#170091Purposeexpresses E. coli type I-E Cas6DepositorInsertE. coli type I-E Cas6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.N
Plasmid#79468PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, NeomycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 608 : pHYG_GFP
Plasmid#92089PurposePlasmid for tagging proteins in Cryptococcus neoformans; GFP tag, HYG selectionDepositorInsertGFP
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SMAD2_HAe2_Bsd
Plasmid#167166PurposeBlasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.DepositorInsertRepair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
UseRepair template for gene editing in human cellsTagsblasticidinExpressionMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP
Plasmid#84255PurposeExpresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent markerDepositorInsertSa sgCXCR4-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP B56 alpha shRNA 6-3
Plasmid#10680DepositorInsertPP2A B56 alpha shRNA (PPP2R5A Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEPYCdKN0001
Plasmid#196148PurposeGateway destination vector for protein expression of 9xHis_AtTGA1_HiBitDepositorInsert9xHis_AtTGA1_HiBit
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D.R
Plasmid#51510PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huLb5Cas12a
Plasmid#174684PurposeExpresses 6xHis-MBP tagged Lb5Cas12aDepositorArticleInserthuLb5Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-30nmSAH-DmrA
Plasmid#61680PurposeGFP-labeled SAH scaffold with DmrA attachment siteDepositorInsertmEGFP-30nmSAH-DmrA
UseTagsExpressionMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.H
Plasmid#79464PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selectionDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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LDB107
Plasmid#185423PurposeNUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporinDepositorInsertNUP1
UseTagsHAExpressionYeastMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-06
Plasmid#119985PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with sfGFPDepositorInsertsfGFP
UsePcr templateTagssfGFPExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only