Addgene

We narrowed to 15,358 results for: LEA

Showing: 2861 - 2880 of 15358 results

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pCRL1

Plasmid

#153997

Purpose

sgRNA in vitro transcription plasmid.

Depositor

Shengdar Tsai

Article

Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

Type

Empty backbone

Use

CRISPR; Sgrna under a t7 promoter for in vitro tr…

Tags

Expression

Mutation

Promoter

T7

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSL4164 (pEffector_IscB,ωRNA)

Plasmid

#210874

Purpose

Expresses GstIscB and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstIscB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-Fn-crRNA

Plasmid

#78958

Purpose

Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAC37

Plasmid

#111618

Purpose

Fluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa

Depositor

Tim Tolker-Nielsen

Article

Yang et al Microbiology. 2007 May;153(Pt 5):1318-28

Insert

PpqsA-gfp(ASV)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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PSUPER-344(hPot1)

Plasmid

#11133

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pBAD/HisB-PhoCl

Plasmid

#87688

Purpose

Express PhoCl in E. coli

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pNuc-TrxRFP1

Plasmid

#98998

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1 and nuclear localization sequence

Use

Tags

His-tag and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBad33_ISTfu1_reRNA

Plasmid

#205355

Purpose

Bacterial expression of ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISTfu1 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-3TM-tevs-AAMP

Plasmid

#179633

Purpose

SEAP RELEASE construct with AAMP motif control

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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mitoZFD_ND1-Right-G1397-C

Plasmid

#180769

Purpose

Expresses mitoZFD_ND1-Right-G1397-C(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Article

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND1 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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mitoZFD_ND1-Left-G1397-N

Plasmid

#180768

Purpose

Expresses mitoZFD_ND1-Left-G1397-N(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Article

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND1 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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HS-mKate2

Plasmid

#105945

Purpose

heat shock inducible transgenesis, cytosolic mKate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

mKate2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBad33_ISMmu1_reRNA

Plasmid

#205349

Purpose

Bacterial expression of ISMmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISMmu1 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISAba30_reRNA

Plasmid

#205323

Purpose

Bacterial expression of ISAba30 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAba30 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBad33_ISDge10_reRNA

Plasmid

#205340

Purpose

Bacterial expression of ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISDge10 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB

Plasmid

#132929

Purpose

Minimal HIV-1 packaging plasmid for gag and pol expression

Depositor

Jeremy Luban

Article

Berthoux et al J Virol. 2004 Nov;78(21):11739-50. doi:

Insert

gag and pol

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-TRE-dCas9-EGFP

Plasmid

#62347

Purpose

Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

dCas9-EGFP

Use

CRISPR

Tags

HA and SV40 NLS

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCASeGFP-pA

Plasmid

#59766

Purpose

Expresses CAS9-eGFP fusion protein in mammalian cells.

Depositor

Wataru Fujii

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

CAS9

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pSL4672 (pEffector_TnpB3,ωRNA)

Plasmid

#210872

Purpose

Expresses GstTnpB3 and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstTnpB3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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