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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 2861 - 2880 of 12310 results

  5. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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  6. pBAD24-rnc-sfGFP

    Plasmid
    #51560
    Purpose
    Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).
    Depositor
    Sankar Adhya, Francisco Malagon
    Publication
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    rnc (rnc E. coli)
    Tags
    GLESTCRHASLAVLADERRFSA and superfolder GFP
    Expression
    Bacterial
    Mutation
    Contains additonal aa at the end of the sfGFP in …
    Available since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51560-plasmid-map-sequence-id-78838

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  7. Monomer NK

    Plasmid
    #31178
    Depositor
    Feng Zhang
    Insert
    Monomer-NK
    Expression
    Bacterial
    Available since
    July 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  8. pcDNA3-GR-AKAR3

    Plasmid
    #173015
    Purpose
    Improved green-red FRET-based PKA activity reporter.
    Depositor
    Jin Zhang
    Publication
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GR-AKAR3
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173015-plasmid-map-sequence-id-346725

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  9. pcDNA3.1-mPCSK7

    Plasmid
    #122676
    Purpose
    Expresses mouse PCSK7 in mammalian cells
    Depositor
    Liming Pei
    Publication
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)
    Expression
    Mammalian
    Promoter
    T7
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122676-plasmid-map-sequence-id-244740

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  10. pHR-pSFFV-TAF15N-mCherry-sspB

    Plasmid
    #122436
    Purpose
    Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.
    Depositor
    Clifford Brangwynne
    Publication
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    TAF15 (TAF15 Human)
    Use
    Lentiviral
    Tags
    mCherry and sspB
    Expression
    Mammalian
    Mutation
    Contains only amino acids 1-208
    Promoter
    SFFV
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    122436-plasmid-map-sequence-id-243727

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  12. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

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  15. pcDNA3.1-mPCSK2

    Plasmid
    #122673
    Purpose
    Expresses mouse PCSK2 in mammalian cells
    Depositor
    Liming Pei
    Publication
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)
    Expression
    Mammalian
    Promoter
    T7
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122673-plasmid-map-sequence-id-244739

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  17. mCer-Vamp2(qv/fw)

    Plasmid
    #53238
    Purpose
    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Mutated Q76 to V; F77 to W.
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Publication
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122174-plasmid-map-sequence-id-237469

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Showing: 2861 - 2880 of 12310 results