We narrowed to 25,849 results for: LEA

1. pMBP-LbCas12a D832A

   Plasmid

   #113517

   Purpose

   Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   D832A

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-T3-eSpCAS9-NG-pA

   Plasmid

   #131466

   Purpose

   Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized eSpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD44I-c013

   Plasmid

   #175450

   Purpose

   Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Tags

   His6

   Expression

   Mammalian

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-T3-SpCAS9-NG-pA

   Plasmid

   #131465

   Purpose

   Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized SpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b I-SceI (Ctermhis -MH)

   Plasmid

   #195613

   Purpose

   Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJS124

   Plasmid

   #207593

   Purpose

   GFP-BIB H20A expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-BIB H20A
   

   Expression

   Bacterial

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmOrange 2-Actin

   Plasmid

   #199703

   Purpose

   visualization of cleavage furrow in living mammalian mitotic cells

   Depositor

   Kenji Sugimoto

   Article

   Tanaka et al Bioimages 25: 7-19, 2017

   Insert

   human cytoplasmic action
   

   Tags

   mOrange 2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJFRC7-UAS-NPRR_dTK

   Plasmid

   #133244

   Purpose

   Fluorescent reporter for neuropeptide release imaging

   Depositor

   David Anderson

   Article

   Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

   Insert

   drosophila tachykinin-fused codon-optimized GCaMP6s
   

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM0553

   Plasmid

   #128989

   Purpose

   LEU2 integration vector of pZF(3BS)-mRUBY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   Leu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57-mcGAS-LL/RK-NLS

    Plasmid

    #185457

    Purpose

    Inducible nuclear cGAS

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHNFI-B2

    Plasmid

    #31405

    Depositor

    Richard Gronostajski

    Article

    Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

    Insert

    Nuclear Factor IB isoform 2 (Nfib Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Not the same as reference seq. See sequence for m…

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMM0530

    Plasmid

    #128987

    Purpose

    Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    SV40NLS-ZiF268DBD-CRY2PHR
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH6Z-806

    Plasmid

    #85255

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046865

    Plasmid

    #153930

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pL8_3xG34-DCAF8L2_X1

    Plasmid

    #191519

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG34-DCAF8L2 X1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL5_3xG22-DCAF8L2_X1

    Plasmid

    #191516

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG22-DCAF8L2 X1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBzCas13b

    Plasmid

    #164862

    Purpose

    Inducible expression of BzCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    Bergeyella zoohelcum Cas13b
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. GPKAnls

    Plasmid

    #61094

    Purpose

    GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal

    Depositor

    Steven Green

    Article

    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

    Insert

    pkac2
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMM0623

    Plasmid

    #128998

    Purpose

    pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMM0624

    Plasmid

    #128999

    Purpose

    pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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