We narrowed to 18,824 results for: nls

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P25: pHR-SFFVp-NLS-FenixS-EGFP-FTH1

Plasmid

#250531

Purpose

Self-assebled 24-mer tagged with NLS, FenixS, and EGFP. Upon red-light activation can bind up to 24 Ash1-modified proteins.

Depositor

Andrew Woolley

Publication

Le et al bioRxiv 2025.11.29.691301

Insert

FenixS

Use

Lentiviral

Tags

EGFP-FTH1 and NLS

Expression

Mammalian

Promoter

SFFV

Available since

Aug. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pDN-D2irTNG4kwh

Plasmid

#44722

Depositor

Gabor Balazsi

Publication

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

pCMV-D2i promoter
htetR::NLS::EGFP

Use

Synthetic Biology; Expression regulator/reporter;…

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pBOBI C-FLAG PHGDH T57A

Plasmid

#212996

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Publication

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Mutation

T57A

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE-EGFP

Plasmid

#89871

Purpose

EGFP reporter vector for iTango system

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tetO-EGFP

Expression

Mammalian

Promoter

TetO

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS

Plasmid

#107327

Purpose

Expresses SpCas9 fused to dNmCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead NmCas9 (D16A, D5…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-YFP-NLS

Plasmid

#145277

Purpose

Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Publication

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HOXD13-IDR (HOXD13 Human)

Expression

Mammalian

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-NLSlacZ-stop-L2

Plasmid

#186358

Purpose

Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ Synthetic, E. coli)

Use

Expression of nuclear lacz reporter

Tags

Nuclear localization signal

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145269

Purpose

Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Publication

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TBP(38Q)-IDR (TBP Human)

Expression

Mammalian

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

Plasmid

#108846

Purpose

Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

Plasmid

#108848

Purpose

Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

pMag,NLS

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

Plasmid

#167832

Purpose

Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

Depositor

Hugo Snippert

Publication

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

HRAS(N17) (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S17N

Promoter

Ubc, TRE

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Plasmid

#159798

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Plasmid

#159799

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)

Plasmid

#107135

Purpose

cGAL-C driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans myo-2 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-mAzGreen-Cyclin D1 (HP)

Plasmid

#215072

Purpose

Expresses NLS-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Publication

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

NLS-mAzGreen

Expression

Mammalian

Mutation

Cyclin D1(HP) denotes mutations M56A, V60A, and W…

Promoter

LTR

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3omega2_[10x 2EBS-S10:35Smp:3x NLS:3x YPet:35Sterm]-[tNOS:BASTA:pNOS]

Plasmid

#254065

Purpose

3x YPet expression driven by 10x 2EBS-S10

Depositor

Jose Alonso, Anna Stepanova

Publication

Fernandez-Moreno et al Plant Biotechnol J. 2024 Jul;22(7):1942-

Insert

10x 2EBS-S10:35Smp:3x NLS:3x YPet:35Sterm

Use

Synthetic Biology

Expression

Plant

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-C2-P2A-mCherry-NLS

Plasmid

#251137

Purpose

Gachapin-C with a transfection marker mCherry-NLS

Depositor

Takeharu Nagai

Publication

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-C2

Tags

P2A-mCherry-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-NLS-Mut

Plasmid

#235206

Purpose

Expresses GRP78 with mutated NLS in mammalian cells

Depositor

Amy Shiu Lee

Publication

Liu et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Mutate the NLS sequence

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Publication

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCR1316

Plasmid

#111126

Purpose

Expresses FANCA.

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA (FANCA Human)

Use

Lentiviral

Tags

HA

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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