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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,508 results for: nls

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Showing: 2861 - 2880 of 6508 results

  5. pT2iC6Dronpa2nlsER4(#636)

    Plasmid
    #184067
    Purpose
    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Dronpa2-2nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184067-plasmid-map-sequence-id-363960

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  19. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139087-plasmid-map-sequence-id-273883

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Showing: 2861 - 2880 of 6508 results