We narrowed to 166,911 results for: DES

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pCAG ERT2-Cre-ERT2 lox

Plasmid

#183447

Purpose

CreOFF tamoxifen-inducible Cre

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

ERT2-Cre-ERT2

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-FAK W266A

Plasmid

#186149

Purpose

For expressing FAK in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIA1414

Plasmid

#172402

Purpose

Expression of SARS-COV-2 Nsp9 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp9 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pREDusk-AmpR-MCS

Plasmid

#188974

Purpose

For red light-repressed bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Ube2v2

Plasmid

#31430

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2V2 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDRF1-GW yoeGFP-T2A-mCherry

Plasmid

#118446

Purpose

Produces equimolar expression of yeast codon-optimized eGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yoeGFP-T2A-mCherry

Expression

Yeast

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pPROK AdoHcyase

Plasmid

#14683

Depositor

Michael Hershfield

Article

Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

Insert

adenosyl-homocysteine hydrolase (AHCY Human)

Expression

Bacterial

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only
pMOD_C'5014

Plasmid

#127219

Purpose

Module C' with Pnos::WUS2

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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rbcLS-pMAL-2px

Plasmid

#41621

Depositor

Ichiro Matsumura

Article

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

rbcL/rbcS

Expression

Bacterial

Promoter

Ptac

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pLV_ADRB2-mTurquoise2-iLID

Plasmid

#161002

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

ADRB2-mTurquoise2-iLID

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Promoter

no promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEXPYR

Plasmid

#70030

Purpose

High yield protein secretion in Aspergillus nidulans

Depositor

Rolf Prade

Article

Segato et al Enzyme Microb Technol. 2012 Jul 15;51(2)

Type

Empty backbone

Use

Synthetic Biology

Promoter

A. niger glucoamylase promoter (glaAp)4

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)

Plasmid

#86874

Purpose

To express MORF4L1 (iso2) with a FLAG tag at N-terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

MORF4L1 (MORF4L1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymTurquoise2-CaURA3

Plasmid

#118458

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection. *An updated version of this plasmid is available: #168057 pFA6a-link-ymTq2-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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px458_Conc3

Plasmid

#134450

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymScarletI-T2A-mTurquoise2

Plasmid

#118441

Purpose

Produces equimolar expression of yeast codon-optimized mScarletI and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymScarletI-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP P495L

Plasmid

#62232

Purpose

Mutant of hamster BiP-mGFP unable to bind clients.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Ube2r1

Plasmid

#31426

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (CDC34 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
Ube2s

Plasmid

#31417

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBA7 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
P1-M-P2

Plasmid

#87091

Purpose

This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pSA293-CHEQ-seq

Plasmid

#174669

Purpose

Reporter plasmid for MPRA containing a SCP1 promoter, a chimeric intron and the Venus cDNA.

Depositor

Stein Aerts

Article

Mauduit et al Elife. 2021 Dec 7;10. pii: 71735. doi: 1

Type

Empty backbone

Use

Enhancer reporter

Promoter

SCP1

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-POLR2G-GFP

Plasmid

#86872

Purpose

To express POLR2G (RPB7) tagged with VENUS at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

POLR2G (POLR2G Human)

Tags

VENUS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pOC4 cloning template vector

Plasmid

#183423

Purpose

FlpON knock-in vector with FLAG-SpCas9 expression

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-AVT1 chr I

Plasmid

#41898

Purpose

Yeast expression of AVT1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

AVT1 (AVT1 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-mUbp43-His

Plasmid

#12458

Depositor

Dong-Er Zhang

Article

Malakhov et al J Biol Chem. 2002 Mar 22. 277(12):9976-8

Insert

UBP43 (Usp18 Mouse)

Tags

GST and His

Expression

Bacterial

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only