We narrowed to 17,230 results for: EMB

1. pAV10.K5.loxP

   Plasmid

   #63204

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV10.HO3.loxP

   Plasmid

   #63205

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV10.HO6

   Plasmid

   #63211

   Purpose

   pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TPC2-D276K-G-GECO1.2

   Plasmid

   #207142

   Purpose

   Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPC2N2 (TPCN2 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Changed Aspartate 276 to Lysine

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-hGal3

   Plasmid

   #73080

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin-3 (LGALS3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Lyn-ICUE3

   Plasmid

   #61623

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   Lyn-ICUE3 (RAPGEF3 Human)
   

   Use

   Tags

   ECFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TPC2-mCherry

   Plasmid

   #135183

   Purpose

   Expresses TPC2 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC2 (Tpcn2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TPC1-mCherry

   Plasmid

   #135182

   Purpose

   Expresses TPC1 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC1 (Tpcn1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-2xFYVE

   Plasmid

   #140047

   Purpose

   Expresses GFP-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG26

    Plasmid

    #226743

    Purpose

    Expresses a reporter for a mitochondrial intermembrane space protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MICU1 (targeting sequence) (MICU1 Human)
    

    Use

    Lentiviral

    Tags

    GFP1-10

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCW57-CMV-MAAP2

    Plasmid

    #218244

    Purpose

    1) Ectopic expression of MAAP2 protein in mammalian cells. 2) Packaging MAAP2 sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein from Adeno-Associated Virus 2 driven by CMV promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

    Plasmid

    #203228

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Insert

    Membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDN34-BiNLS-hYAP1-1delta

    Plasmid

    #169471

    Purpose

    expresses optoYAP in mammalian cells

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    YAP1-1delta (YAP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMP71-tCD19-mGFP

    Plasmid

    #174609

    Purpose

    expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    CD19 extracellular and transmembrane domain (Cd19 Mouse)
    

    Use

    Tags

    palmitoylated EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1/CIP2A(1-905) WT V5 His

    Plasmid

    #119287

    Purpose

    CIP2A WT V5 overexpression

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQFlag-USP7 WT puroR

    Plasmid

    #46751

    Purpose

    Retroviral vector that expresses wild type Flag-tagged human USP7

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GST PTBP1 isoform 4

    Plasmid

    #108594

    Purpose

    Recombinant GST tagged protein production

    Depositor

    Chris Smith

    Article

    Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

    Insert

    PTBP1 isoform 4 (PTBP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    isoform 4 (isoform a)

    Promoter

    tac

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSCIP-hCD69-TdTomato

    Plasmid

    #154095

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Inserts

    [5HA]-P2A-tdTomato-[3HA]
    hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT-TO-NOD1-EGFP

    Plasmid

    #131206

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD1 (NOD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-p53 2KR

    Plasmid

    #78799

    Purpose

    To overexpress p53 2KR in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K291R, K292R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only