We narrowed to 17,410 results for: EMB

1. GFP-Miro1 (184-618)

   Plasmid

   #127626

   Purpose

   Human Miro1 lacking the first GTPase domain

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   184-618 only

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FB010

   Plasmid

   #119708

   Purpose

   Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   PtrpC:hph:Ttub
   

   Use

   Synthetic Biology

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. NET39 Short pmRFP-N2 (451)

   Plasmid

   #61981

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET39 (PPAPDC3 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Short form of NET39

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. CC1

   Plasmid

   #63713

   Purpose

   Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain

   Depositor

   Bradley Baker

   Article

   Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

   Insert

   CC1
   

   Tags

   Super ecliptic pHlorin A227D

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMM2-hPMCA4bct120

   Plasmid

   #47760

   Purpose

   mammalian expression of human PMCA4 with C-terminal 120aa deletion

   Depositor

   Emanuel Strehler, John Penniston

   Article

   Enyedi et al J Biol Chem. 1993 May 15;268(14):10621-6

   Insert

   PMCA4bct120 (ATP2B4 Human)
   

   Expression

   Mammalian

   Mutation

   deletion of C-terminal 120 aa (removes calmodulin…

   Promoter

   SV40

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pIRES:hTRAAK(W262S)

   Plasmid

   #133078

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
   

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES:hTRAAK(G124I)

   Plasmid

   #133079

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
   

   Expression

   Mammalian

   Mutation

   G124I

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF(UG) hSyn NS34a-msGFP-A'Q-mito

   Plasmid

   #158766

   Purpose

   Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Lentiviral

   Mutation

   Flexible linkers between the protease and msGFP a…

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF(UG) hSyn NS34a-msGFP-'Q-mito

   Plasmid

   #158767

   Purpose

   Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Lentiviral

   Mutation

   Flexible linkers between the protease and msGFP a…

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAV10.K3.loxP

    Plasmid

    #63202

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV10.K6.loxP

    Plasmid

    #63203

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV10.K5.loxP

    Plasmid

    #63204

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV10.HO3.loxP

    Plasmid

    #63205

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV10.HO6

    Plasmid

    #63211

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Lyn-ICUE3

    Plasmid

    #61623

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    Lyn-ICUE3 (RAPGEF3 Human)
    

    Tags

    ECFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-hGal3

    Plasmid

    #73080

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin-3 (LGALS3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-EGFP-eDHFR(69K6)-MCS

    Plasmid

    #172100

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    EGFP-eDHFR(69K6)-20aa
    

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TPC2-mCherry

    Plasmid

    #135183

    Purpose

    Expresses TPC2 with C-terminus mCherry tag in mammalian cells

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP VARP

    Plasmid

    #42312

    Depositor

    Thierry Galli

    Article

    Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

    Insert

    VARP (ANKRD27 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only