We narrowed to 7,305 results for: plasmid dna
-
Plasmid#108242PurposeLexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.DepositorInsertLexA DNA binding domain fusing with VHH-Caffeine antibody
UseSynthetic BiologyPromoterpLacO1Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
AY22_pgRNA.R5.1
Plasmid#100294PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6 RNA Pol III promoter human snRNAAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag-P2A-tagBFP
Plasmid#174141PurposeExpresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.DepositorInsertdCas9, 10xSunTag, tagBFP
TagsHA and tagBFPExpressionMammalianPromoterSV40Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PAmCherry-RPA194
Plasmid#247354PurposeCRISPR/Cas9 donor plasmid to tag human RPA194 with PAmCherryDepositorAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLP-mScarlet-I
Plasmid#209993PurposeContains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmidsDepositorInsertmScarlet-I
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-hU6-evopreQ1
Plasmid#210189PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAP118
Plasmid#105495PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusionsDepositorInsertHupA-SmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficilePromoterPtetAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-greenlantern
Plasmid#202141PurposeLevel 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertGreen lantern gene
UseSynthetic BiologyMutationNoneAvailable SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
LentiV2-mU6-evopreQ1
Plasmid#210190PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICE-EGFP-FLAG-Ku70siR-WT
Plasmid#46961PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-SsAPOBEC3B
Plasmid#138343PurposeMammalian expression plasmid for BE4-SsAPOBEC3BDepositorInsertBE4-SsAPOBEC3B
UseSynthetic BiologyTagsBP-NLSPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2
Plasmid#167296PurposePlasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFPDepositorInsertBAX (BAX Human)
ExpressionMammalianAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFPbait-E2A-KalTA4-pA donor vector
Plasmid#61069Purposefor CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)DepositorInsertseGFPbait
KalTA4
UseZebrafish expressionTagsE2AAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-HA-hTet1CD-WPRE-PolyA
Plasmid#39454DepositorInsertHA-hTet1CD (TET1 Human)
UseAAVTagsHAExpressionMammalianMutationaa1418–2136PromoterEF1aAvailable SinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-PpAPOBEC1
Plasmid#138349PurposeMammalian expression plasmid for BE4-PpABOBEC1DepositorInsertBE4-PpAPOBEC1
TagsBP-NLSExpressionMammalianPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His-VCre
Plasmid#123131PurposeBacterial expression plasmid for wild-type VCre with an N-terminal His-tag.DepositorInsertVCre recombinase
UseCre/LoxTags6x His tagExpressionBacterialPromoterT7Available SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGK111
Plasmid#228126PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
ExpressionMammalianPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-AmAPOBEC1
Plasmid#138342PurposeMammalian expression plasmid for BE4-AmABOBEC1DepositorInsertBE4-AmAPOBEC1
UseSynthetic BiologyTagsBP-NLSPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only