We narrowed to 75,907 results for: Rest

1. SFFV-DTAG-NTERM-GFP

   Plasmid

   #185760

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Expression

   Mutation

   none

   Promoter

   SFFV

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGK-HALO-CTERM-GFP

   Plasmid

   #185777

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, Halo

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PGK-AID-CTERM-GFP

   Plasmid

   #185778

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, AID

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SFFV-IKZF3-CTERM-GFP

   Plasmid

   #185769

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, IKZF3 minidegron

   Expression

   Mutation

   none

   Promoter

   SFFV

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PGK-SMASH-NTERM-GFP

   Plasmid

   #185771

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, SMASh

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PGK-HALO-NTERM-GFP

   Plasmid

   #185772

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, Halo

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCI-neoBeta2mcherry

   Plasmid

   #45097

   Depositor

   Henry Lester

   Article

   Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

   Insert

   nAChR beta2 mcherry (Chrnb2 Mouse)
   

   Use

   Tags

   has c-myc tag

   Expression

   Mammalian

   Mutation

   β2 was cloned into pCI-neo at the restriction sit…

   Promoter

   CMV

   Available since

   June 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SFFV-DTAG-CTERM-GFP

   Plasmid

   #185765

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Expression

   Mutation

   none

   Promoter

   SFFV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PGK-IKZF3-NTERM-GFP

    Plasmid

    #185774

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    William Sellers, Alessandra Ianari

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, IKZF3 minidegron

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HeLa full-length MLCK-GFP

    Plasmid

    #46316

    Depositor

    Anne Bresnick

    Article

    Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

    Insert

    HeLa long myosin light chain kinase (MYLK Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PGK-AID-NTERM-GFP

    Plasmid

    #185773

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    William Sellers, Alessandra Ianari

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, AID

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_Ef1a:PE2-P2A-hMLH1dn

    Plasmid

    #184444

    Purpose

    Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ZNF217_pLX307

    Plasmid

    #98384

    Purpose

    Lentiviral expression of ZNF217

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    ZNF217 (ZNF217 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SFFV-IKZF3-NTERM-GFP

    Plasmid

    #185764

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    William Sellers, Alessandra Ianari

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, IKZF3 minidegron

    Expression

    Mutation

    none

    Promoter

    SFFV

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-EGFP-eDHFR(69K6)-MCS

    Plasmid

    #172100

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    EGFP-eDHFR(69K6)-20aa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Use

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Syn-pAce-Kv2.1PR

    Plasmid

    #195523

    Purpose

    Green fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAce-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 78K, 81D, 92N, 178F; SY linker

    Promoter

    Synapsin

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SFFV-HALO-NTERM-GFP

    Plasmid

    #185762

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    William Sellers, Alessandra Ianari

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, Halo

    Expression

    Mutation

    none

    Promoter

    SFFV

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid

    #184379

    Purpose

    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Tet3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge