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NST1_pOpOn2.1

Plasmid

#228065

Purpose

Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

Depositor

Charles Anderson

Article

Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

Insert

NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)

Use

Dexamethasone inducible vector

Expression

Plant

Promoter

CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-ArcFL

Plasmid

#119877

Purpose

Bacterial Expression construct for protein production and purification

Depositor

Jason Shepherd

Article

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Expression

Bacterial

Promoter

tac

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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CDK2_HUMAN_D0

Plasmid

#79726

Purpose

This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDK2 (CDK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DYR1A_HUMAN_D0

Plasmid

#79690

Purpose

This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK1A (DYRK1A Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-eGFP-hcGAS

Plasmid

#108674

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (CGAS Human)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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SRC_HUMAN_D0

Plasmid

#79700

Purpose

This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

SRC (SRC Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-opCas12a-6xNLS

Plasmid

#209022

Purpose

Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

opCas12a-6xNLS-Flag-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

FLAG and MYC

Expression

Mammalian

Mutation

E174R/S542R

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-UltraCas12a-6xNLS

Plasmid

#209023

Purpose

Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

UltraCas12a-6xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

M537R/F870L

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2-SBECN1W

Plasmid

#107937

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

mouse Beclin-1 (BECN1) (Becn1 Mouse)

Use

AAV

Promoter

hSynapsin

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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PET01-TAF5-ex8-Minigene-SRSF1 motif WT

Plasmid

#218972

Purpose

Vector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8

Expression

Mammalian

Promoter

RSV LTR

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-NoABE

Plasmid

#209043

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
TMEM216-mCherry

Plasmid

#41633

Depositor

Joseph Gleeson

Article

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 216 (TMEM216 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
pEMS2241

Plasmid

#111896

Purpose

ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

AAV

Promoter

Ple320 (POU4F1 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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3xflag-APEX2-LRRK2

Plasmid

#164622

Purpose

Expresses LRRK2 tagged with APEX2 and 3xFLAG

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

LRRK2 (LRRK2 Human)

Tags

3xFLAG and APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM45_WT

Plasmid

#82892

Purpose

Gateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS2280

Plasmid

#111901

Purpose

ssAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple345 (NEFL Human)

Use

AAV

Promoter

Ple345 (NEFL MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAED2

Plasmid

#234599

Purpose

Fluorescent reporter of the SOS response

Depositor

Tanel Tenson

Article

Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):

Inserts

mScarlet-I
gfp-mut2

Use

Reporter

Expression

Bacterial

Mutation

GFPmut2 was derived from avGFP with the following…

Promoter

Ptet+dnaK P1 and cda

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-enCas12a-2xNLS

Plasmid

#209021

Purpose

Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

enCas12a-2xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

E174R/S542R/K548R

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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