We narrowed to 31,396 results for: Eng

1. Ef1a_Large T-antigen_Ires_Puro

   Plasmid

   #18922

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   Large T Antigen (SV40gp6 Simian virus 40)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_AAVS1-T2

   Plasmid

   #41818

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. Reporter-1

   Plasmid

   #180218

   Purpose

   Surrogate reporter plasmid for the assay of targeted RNA editing

   Depositor

   Wensheng Wei

   Article

   Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

   Insert

   Surrogate reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Reporter-2

   Plasmid

   #180219

   Purpose

   Surrogate reporter plasmid for the assay of targeted RNA editing

   Depositor

   Wensheng Wei

   Article

   Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

   Insert

   Surrogate reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAJE-Ma pylRS nitroY/haloY-F5

   Plasmid

   #225684

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

   Depositor

   Ryan Mehl

   Inserts

   M. alvus PylRS 3-NY/HaloY F5
   M. alvus Pyl-tRNA(6)
   

   Use

   Methanomethylophilus alvus

   Tags

   Expression

   Mutation

   CTG125CTT, N166A, V168S, A223C, W239R

   Promoter

   GlnS and Lpp

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p177bp-LacOx50-Hyg

   Plasmid

   #210213

   Purpose

   Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiSAMPHv2

   Plasmid

   #167934

   Purpose

   All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/TO-cMYC

    Plasmid

    #216510

    Purpose

    Expresses MYC in human cells under a tetracycline/doxycycline-inducible promoter

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    cMYC
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAct-Cas9

    Plasmid

    #62209

    Purpose

    Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-GfaABC1D-GRAB_Ado1.0

    Plasmid

    #153288

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EFS-DIO-GRAB_Ado1.0

    Plasmid

    #153291

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSH62-EBD

    Plasmid

    #49455

    Purpose

    yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase

    Depositor

    Marc Gartenberg

    Article

    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

    Insert

    Cre-EBD fusion
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1 promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRGEB33

    Plasmid

    #158151

    Purpose

    Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORF1-2

    Plasmid

    #206024

    Purpose

    An expression vector for mouse LINE1 ORF1-2

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF1-2 derived from mouse LINE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. coel::RFP

    Plasmid

    #8938

    Depositor

    Piali Sengupta

    Article

    Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

    Insert

    unc-122 promoter (unc-122 Nematode)
    

    Use

    Tags

    RFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHelper_TetR/pTet-Tnpase (camR)

    Plasmid

    #213910

    Purpose

    ATc-inducible himar1C9 transposase expression - cam resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC-SP6

    Plasmid

    #102418

    Purpose

    Ac transposase with SP6 promoter for in vitro transcription

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    Ac transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP6

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP_sfCherry2(11)_H2B

    Plasmid

    #82605

    Purpose

    Expresses sfCherry2(11) tagged H2B in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pORF2

    Plasmid

    #206026

    Purpose

    An expression vector for mouse LINE1 ORF2

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF2 derived from mouse LINE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP_TOMM20_sfCherry2(11)

    Plasmid

    #83033

    Purpose

    Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    TOMM20_sfCherry+11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVC-Ds-MCS-Ds

    Plasmid

    #102416

    Purpose

    Empty vector with a multiple cloning site flanked by Ds integration elements

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LES2A-CRE

    Plasmid

    #167933

    Purpose

    Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    mScarlet-P2A-CRE
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pME-cas9-2A-mCherry

    Plasmid

    #107592

    Purpose

    An entry vector with cas9 -2A-mCherry

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    cas9-2A-mCherry
    

    Use

    CRISPR

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pre_II_CS3_9072_v1

    Plasmid

    #126854

    Purpose

    9072 b scaffold from compatibility group 3, made by method II

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_9072_v1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid

    #41863

    Purpose

    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge