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pHis17_ParM _1

Plasmid

#78201

Purpose

Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

Depositor

Martin Webb

Article

Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

Insert

parM (K33A I27C T174A T175N C287A)

Tags

His6

Expression

Bacterial

Mutation

K33A I27C T174A T175N C287A

Promoter

unknown

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pExp_CAG:EBFP2

Plasmid

#51790

Purpose

Constitutively expresses blue fluorescent protein, EBFP2

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

EBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pSEVA13-GG

Plasmid

#208002

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pICH47732:FCP:NAT

Plasmid

#85984

Purpose

Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

NAT

Use

Golden gate assembly

Promoter

FCP

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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HaloKu70 sgRNA

Plasmid

#207583

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

GAGCAGTAGCCAACATGTCA

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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hSgk1_v2 Flag

Plasmid

#86040

Purpose

full length human Sgk1_i2 with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

Insert

Alternate transcript v2 of human Sgk1 (SGK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

v2

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pKT-iRFP-KAN

Plasmid

#64687

Purpose

pKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

iRFP

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pNIC-AncCAR_A

Plasmid

#168286

Purpose

Expresses AncCAR_A in E. coli from a T7 promoter

Depositor

Nicholas Harmer

Article

Thomas et al Commun Biol. 2019 Nov 22;2:429. doi: 10.

Insert

AncCAR_A

Use

Synthetic Biology

Tags

6*His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-RPA43

Plasmid

#17659

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA43 (Twistnb Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGL193

Plasmid

#180471

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Cas9

Expression

Plant

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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Venus-giantin

Plasmid

#234749

Purpose

Mammalian expression of Golgi marker Venus-giantin

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-giantin

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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Venus-MOA

Plasmid

#234741

Purpose

Mammalian expression of mitochondria marker Venus-MOA

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-MOA

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEYFP-p23

Plasmid

#108225

Purpose

Mammalian expression vector for EYFP fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

EYFP-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK055

Plasmid

#171327

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

rTA2S-M2 (TetR-3xVP16) transcriptional activator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K-CAAX

Plasmid

#41644

Purpose

Expresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box)

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145K-CAAX

Tags

CAAX box

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCalpha EV (PKA catalytic subunit Calpha)

Plasmid

#15310

Depositor

Stanley McKnight

Article

Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

Insert

PRKACA (Prkaca Mouse)

Expression

Mammalian

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
CMV-Brainbow-1.0 H

Plasmid

#18720

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

tdimer2 ; M-mEYFP ; M-mCerulean

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-beta-arrestin2-HA-TEV219

Plasmid

#104845

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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