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OMM-long-CFAST10

Plasmid

#233598

Purpose

Expression of CFAST10 on the OMM membrane after a long linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 CS puroR

Plasmid

#46752

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-mGFP

Plasmid

#174609

Purpose

expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD19 extracellular and transmembrane domain (Cd19 Mouse)

Tags

palmitoylated EGFP

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NOD1-EGFP

Plasmid

#131206

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD1 (NOD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-ICUE3

Plasmid

#61623

Purpose

Plasma membrane-targeted FRET biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

Lyn-ICUE3 (RAPGEF3 Human)

Tags

ECFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP-shTACC3

Plasmid

#59356

Purpose

Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 30-33 to confer s…

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shControl

Plasmid

#180386

Purpose

Producing AAV that encodes negative control shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shControl

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-DIO-Lck-mCherry-ibARK-4x6T

Plasmid

#241243

Purpose

Cre-dependent expression plasma membrane-tethered ibARK with higher specificity in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

DIO-Lck-mCherry-ibARK-4x6T

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP VARP

Plasmid

#42312

Depositor

Thierry Galli

Article

Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

Insert

VARP (ANKRD27 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only
OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203229

Purpose

Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Cre-dependent, membrane-localized Optopatch

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pX330a dCas9-LSD1

Plasmid

#92362

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-LSD1

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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TPC2-mCherry

Plasmid

#135183

Purpose

Expresses TPC2 with C-terminus mCherry tag in mammalian cells

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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TPC1-mCherry

Plasmid

#135182

Purpose

Expresses TPC1 with C-terminus mCherry tag in mammalian cells

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC1 (Tpcn1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

Plasmid

#188994

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-eDHFR(69K6)-MCS

Plasmid

#172100

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)-20aa

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAG26

Plasmid

#226743

Purpose

Expresses a reporter for a mitochondrial intermembrane space protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MICU1 (targeting sequence) (MICU1 Human)

Use

Lentiviral

Tags

GFP1-10

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBeta-MHC-promoter DV (JDW 26)

Plasmid

#229835

Purpose

A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pQHA-USP7 WT puroR

Plasmid

#46753

Purpose

Retroviral vector that expresses wild type HA-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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mCer-4aa-VAMP2

Plasmid

#53233

Purpose

Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

VAMP2 (Vamp2 Rat)

Tags

4aa-linker and mCerulean

Expression

Mammalian

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only