Addgene

We narrowed to 323 results for: pcl

Showing: 281 - 300 of 323 results

Results per page:

Sort results by:

pGTMV

Plasmid

#118755

Purpose

Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.

Depositor

Jose-Antonio Daros

Article

Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

Insert

Tobacco mosaic virus

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM12

Plasmid

#101643

Purpose

Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meffCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM17

Plasmid

#101641

Purpose

Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

eqFP486

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM32.2

Plasmid

#101635

Purpose

Encodes the fluorescent protein Rosmarinus under a CMV promoter.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Rosmarinus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM14

Plasmid

#101645

Purpose

Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

efasCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM32

Plasmid

#101634

Purpose

Encodes the fluorescent protein Rosmarinus for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Rosmarinus

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pThom77.2

Plasmid

#101646

Purpose

Encodes the fluorescent protein amFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CLIPf-KIF5A-SNAPf-HisTag-FLAG

Plasmid

#200789

Purpose

Mammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
KIF5A-SNAPf-HisTag-FLAG

Plasmid

#200791

Purpose

Mammalian expression of KIF5A-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CLIPf-KIF5B-SNAPf-HisTag-FLAG

Plasmid

#200799

Purpose

Mammalian expression of CLIPf-KIF5B-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5B (KIF5B Human)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM13

Plasmid

#101644

Purpose

Encodes the fluorescent protein meleCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meleCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-Tam1

Plasmid

#101639

Purpose

Encodes the fluorescent protein Tam1 for arabinose-inducible expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Tam1

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pThom77.6

Plasmid

#101637

Purpose

Encodes the fluorescent protein amFP486/K68M under a CMV promoter.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486/K68M

Use

Tags

Expression

Mammalian

Mutation

K68M

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pThom77.3

Plasmid

#101636

Purpose

Encodes the fluorescent protein amFP486/K68M for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486/K68M

Use

Tags

6XHis

Expression

Bacterial

Mutation

K68M

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-RBP-LOV2

Plasmid

#185792

Purpose

Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

Depositor

Lizhong He

Article

Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

Insert

RBP-LOV2

Use

Tags

10xHis, TEV protease cleavage site after His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Article

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
RALYL-1

Plasmid

#155877

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RALYL (RALYL Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Plasmid

#200792

Purpose

Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Δ954-1027

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FLT4

Plasmid

#116745

Purpose

Lentiviral expression of FLT4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT4 (FLT4 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge