Skip to main content
Addgene
Showing: 561 - 600 of 13111 results
  1. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
    Covid-SARS2 Nsp10
    Use
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pCVSN2c-MCP-oScarlet-KASH-6xMS2 Barcode Library

    Pooled Library
    #227672
    Purpose
    RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of CVS N2c strain G-deleted rabies virus
    Expression
    Mammalian
    Species
    Synthetic
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

    Plasmid
    #183080
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Insert
    TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    -124C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid
    #183081
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Insert
    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    -146C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid
    #183079
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Insert
    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    Wildtype promoter, Exon 1 C7C (C21>T21) silent…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pAcGFP-IVT

    Plasmid
    #205145
    Purpose
    IVT template of AcGFP with MCS in frame and polyA
    Depositor
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pMDW139

    Plasmid
    #227336
    Purpose
    Template for in vitro Pol II transcription
    Depositor
    Insert
    Gap DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pAcGFP-stop-IVT

    Plasmid
    #205146
    Purpose
    IVT template of AcGFP stop codon between the ORF and MCS and polyA
    Depositor
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pFFLuc-IVT

    Plasmid
    #205147
    Purpose
    IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Insert
    FireFly luciferase
    Use
    Luciferase; In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pRLuc-IVT

    Plasmid
    #205148
    Purpose
    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Insert
    Renilla Luciferase
    Use
    Luciferase; In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. pOME0239M

    Plasmid
    #182601
    Purpose
    Reverse complement antiRTA3kb pOME0238 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Insert
    Reverse compliment of antiRTA3kb
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pOME0241M

    Plasmid
    #182603
    Purpose
    Reverse complement T15 pOME0240 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Insert
    Reverse compliment of T15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pBR HPV31 9E

    Plasmid
    #221009
    Purpose
    Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322
    Depositor
    Insert
    Human papillomavirus type 31 genome 9E isolate
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. pOME0245M

    Plasmid
    #182607
    Purpose
    Reverse complement PAN-RNA pOME0244 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Insert
    Reverse compliment for PAN_RNA (pOME0244)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pOME0243M

    Plasmid
    #182605
    Purpose
    Reverse complement antiRTA1kb pOME0242 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Insert
    Reverse compliment of antiRTA1kb (pOME0242)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pOME0237M

    Plasmid
    #182599
    Purpose
    Reverse complement sequence of anti-PAN pOME0236 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Insert
    Reverse compliment of anti-PAN (pOME0236)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. UQ6106

    Bacterial Strain
    #37176
    Depositor
    Bacterial Resistance
    Ampicillin
    Available since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
  19. pMDW133

    Plasmid
    #227248
    Purpose
    Lambda DNA for torsional templates
    Depositor
    Insert
    Lambda DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. PAS_HeSAHS4

    Plasmid
    #215648
    Purpose
    Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli
    Depositor
    Insert
    SAHS4
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 561 - 600 of 13111 results