Addgene

We narrowed to 878 results for: RK2

Showing: 561 - 600 of 878 results

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pGEX-parkin L102K

Plasmid

#227359

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin L102K (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin M458L

Plasmid

#227356

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin M458L (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin R256C

Plasmid

#227355

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin R256C (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin R234Q

Plasmid

#227354

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin R234Q (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin V224A

Plasmid

#227353

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin V224A (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin P37L

Plasmid

#227352

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin P37L (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET 15b+His SUMO Rab12 (Q101L)

Plasmid

#208371

Purpose

Bacterial expression of His-SUMO-tagged human Rab12 (Q101L)

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

His SUMO Rab12 (Q101L) (RAB12 Human)

Use

Tags

Expression

Mammalian

Mutation

Q101L

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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sgLacZ-puro

Plasmid

#199635

Purpose

sgRNA control guide targeting bacterial LacZ

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgCTRL-puro

Plasmid

#199634

Purpose

sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mPHB2-2

Plasmid

#198484

Purpose

lentiviral stable expression of mPHB2 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mPhb2 (Phb2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 1

Plasmid

#198501

Purpose

lentiviral stable expression of mNudcd3 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-guide-puro mNudcd3 - 2

Plasmid

#198502

Purpose

lentiviral stable expression of mNudcd3 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mSptlc2 - 2

Plasmid

#198494

Purpose

lentiviral stable expression of mSptlc2 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mSptlc2 (Sptlc2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mPHB2-1

Plasmid

#198483

Purpose

lentiviral stable expression of mPHB2 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mPhb2 (Phb2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-2

Plasmid

#198476

Purpose

lentiviral stable expression of mRab12 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-1

Plasmid

#198475

Purpose

lentiviral stable expression of mRab12 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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VRK1-dTag-puro

Plasmid

#199651

Purpose

Expresses VRK1 C-term. Tagged with dTAG

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Tags

dTAG

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN-PAmCherry-KFERQ-NE

Plasmid

#102365

Purpose

Lentiviral overexpression of PA-mCherry-KFERQ fusion

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Inserts

PA-mCherry
KFERQ peptide

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-mSTING

Plasmid

#229739

Purpose

Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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2xmyc-JIP4

Plasmid

#164633

Purpose

Expresses JIP4 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Use

Tags

2xmyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin-A92mKO2

Plasmid

#213559

Purpose

Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK EGFP-PPM1H-WT

Plasmid

#200084

Purpose

Mammalian expression of wild-type PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H (PPM1H Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO PPM1H

Plasmid

#207333

Purpose

Bacterial expression of His SUMO PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK1-K179E

Plasmid

#199644

Purpose

Expresses kinase-inactive VRK1 (K179E)

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

K179E

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCas9-sgVRK1 #4-Blast

Plasmid

#199647

Purpose

Expresses Cas9 and sgRNA guide targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-guide-puro mMyh9 - 2

Plasmid

#198492

Purpose

lentiviral stable expression of mMyh9 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mMyh9 (Myh9 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-guide-puro mMyh9 - 1

Plasmid

#198491

Purpose

lentiviral stable expression of mMyh9 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mMyh9 (Myh9 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-JIP4

Plasmid

#164620

Purpose

Expresses JIP4 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Use

Tags

cycle 3 GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK ARF6(Q67L)-CFP

Plasmid

#200428

Purpose

Mammalian expression of Q67L mutation in ARF6

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

ARF6 (ARF6 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Q67L

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCB306-GFP-Rab8A

Plasmid

#198470

Purpose

lentiviral expression of GFP-Rab8A

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab8A (RAB8A Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-JIP4

Plasmid

#164621

Purpose

Expresses JIP4 in mammalian cells with a mNeonGreen tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCas9-sgVRK1 #1-Blast

Plasmid

#199646

Purpose

Expresses Cas9 and sgRNA guide targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CSII-PPM1H-mApple

Plasmid

#198473

Purpose

Mammalian expression of PPM1H-mApple

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

PPM1H-mApple (PPM1H Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPPC011

Plasmid

#171143

Purpose

Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Sp.pCas9, BBa_J23107

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN Parkin

Plasmid

#89299

Purpose

For expression of untagged Parkin

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CSII-PPM1H D288A-mApple

Plasmid

#198474

Purpose

Mammalian expression of PPM1H-mApple

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

PPM1H-D288A-mApple (PPM1H Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21b GFP-Rab10 Q68LHis

Plasmid

#186015

Purpose

GFP-Rab10 Q68LHis

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

GFP-Rab10 Q68LHis (RAB10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Parkin-A92mKO2

Plasmid

#213548

Purpose

Transient mammalian expresssion of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14b His Rab8a Q67L

Plasmid

#186014

Purpose

His Rab8a Q67L

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

His Rab8a Q67L (RAB8A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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