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Showing: 281 - 300 of 10930 results

  3. pLKO_HA.V5.FLAG_NGFR

    Plasmid
    #158250
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.FLAG
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158250-plasmid-map-sequence-id-327836

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  6. p663-UBC-V5-miniTurbo-V5-STOP_IDG-K

    Plasmid
    #158158
    Purpose
    Gateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    158158-plasmid-map-sequence-id-314579

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  11. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158408-plasmid-map-sequence-id-330919

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  14. pLIX403-EBFP2-ccdB-NatMx

    Plasmid
    #158559
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158559-plasmid-map-sequence-id-309875

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  15. pDOC-K-glmS-GFPfor

    Plasmid
    #158059
    Purpose
    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    Green Fluorescent Protein optimised for excitation with UV light
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    acpP
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158059-plasmid-map-sequence-id-309439

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  20. pAAV-Syn-SomaGCaMP6f1

    Plasmid
    #158755
    Purpose
    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f1
    Use
    AAV
    Expression
    Mammalian
    Promoter
    synapsin promoter
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158755-plasmid-map-sequence-id-310657

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Showing: 281 - 300 of 10930 results