Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 10,401 results for: 158

Showing: 281 - 300 of 10401 results

  1. pYPQ136-tRNA2.0

    Plasmid
    #158398
    Purpose
    Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158398-plasmid-map-sequence-id-330906

    Enlarge

  5. pLKO_HA.NWS.VSVg_NGFR

    Plasmid
    #158243
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158243-plasmid-map-sequence-id-329479

    Enlarge

  6. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158408-plasmid-map-sequence-id-330919

    Enlarge

  10. pDONR221-APN

    Plasmid
    #158440
    Purpose
    Gateway Cloning compatible entry vector for the human APN gene.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    APN (ANPEP Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158440-plasmid-map-sequence-id-308255

    Enlarge

  12. pDONR221-ACE2

    Plasmid
    #158438
    Purpose
    Gateway Cloning compatible entry vector for the human ACE2 gene.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    ACE2 (ACE2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158438-plasmid-map-sequence-id-308248

    Enlarge

  13. pLIX403-ccdB-EYFP

    Plasmid
    #158564
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158564-plasmid-map-sequence-id-309879

    Enlarge

  16. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid
    #158245
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158245-plasmid-map-sequence-id-327706

    Enlarge

  17. pAAV-Syn-SomaGCaMP6f1

    Plasmid
    #158755
    Purpose
    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158755-plasmid-map-sequence-id-310657

    Enlarge

  18. p663-UBC-miniTurbo-V5-CDK2_IDG-K

    Plasmid
    #158193
    Purpose
    Gateway destination clone of CDK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK2 (CDK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    158193-plasmid-map-sequence-id-373670

    Enlarge

  19. pLIX403-EYFP-ccdB-Blast

    Plasmid
    #158551
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158551-plasmid-map-sequence-id-309826

    Enlarge

Showing: 281 - 300 of 10401 results