We narrowed to 30,796 results for: Ide

1. TetOn-CLIMP63-mCherry-eGFP

   Plasmid

   #139871

   Purpose

   Dox-inducible lentiviral expression of human CLIMP63

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   CLIMP63 (CKAP4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dual-CRISPR-Cas9-U6-H1 (pBP48)

   Plasmid

   #218932

   Purpose

   The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   2A-Puro and 3XHA

   Expression

   Mammalian

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-SasgRNA scaffold-CMV-nSaCas9

   Plasmid

   #171695

   Purpose

   Expresses nSaCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   sgRNA scaffold-nSaCas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CMV

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. OPEN-HLA-B*07:02-BirA

   Plasmid

   #239752

   Purpose

   E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-B*07:02 (HLA-B Human)
   

   Tags

   BirA substrate peptide

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   T7

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_502_sgCD45

   Plasmid

   #158705

   Purpose

   CD45 CRISPRa control

   Depositor

   David Root, John Doench

   Article

   Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

   Insert

   CD45 (PTPRC Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-X1-Neo-DDRGK1-WT-HA

   Plasmid

   #139845

   Purpose

   Lentiviral expression of human DDRGK1-WT-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

   Plasmid

   #176540

   Purpose

   Mammalian expression of nuclear localized OgeuIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   OgeuIscB
   

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #159281

    Purpose

    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    AsCpf1-HA-2A-GFP
    

    Use

    Multicistronic vector with both caggs promoter-dr…

    Tags

    3X HA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 proHB-EGF NC

    Plasmid

    #11605

    Depositor

    Rosalyn Adam

    Article

    Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

    Insert

    HB-EGF (HBEGF Human)
    

    Expression

    Mammalian

    Mutation

    Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

12. CFP-NS1

    Plasmid

    #85739

    Purpose

    expresses CFP-FLAG-tagged NS1 monobody.

    Depositor

    John O'Bryan

    Article

    Spencer-Smith et al Nat Chem Biol. 2016 Nov 7. doi: 10.1038/

    Insert

    FLAG-tagged NS1 monobody
    

    Tags

    ECFP and Flag

    Expression

    Mammalian

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PDIA3-bio-His

    Plasmid

    #52070

    Purpose

    Expresses full-length Protein disulfide-isomerase A3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PDIA3 (PDIA3 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT-DestFLAGHA_RBPMS

    Plasmid

    #65743

    Purpose

    constitutively expresses RBPMS in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

15. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-B-evoSpCas9-NLS-6xHis

    Plasmid

    #207390

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc-His-hAR-2

    Plasmid

    #89091

    Purpose

    baculovirus transfer vector for histidine-tagged full-length human androgen receptor, starts Met-Ala-His6-Met-hAR

    Depositor

    Elizabeth Wilson

    Article

    Liao et al Biochemistry. 1999 Jul 27;38(30):9718-27

    Insert

    hAR (AR Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pFRT-DestFLAGHA_RBPMS2

    Plasmid

    #65748

    Purpose

    constitutively expresses FLAG-HA-tagged RBPMS2 in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

19. MSP2135

    Plasmid

    #72248

    Purpose

    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, and D1135E

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-spTorA-GFP-H6C

    Plasmid

    #168517

    Purpose

    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    spTorA-GFP-H6C
    

    Tags

    6xHisC tag and spTorA (signal peptide of E. coli …

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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