We narrowed to 11,628 results for: Vars

1. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

   Plasmid

   #175526

   Purpose

   Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag11 and Tomm20-HaloTag7
   

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpCas9-P2A-puro

   Plasmid

   #214358

   Purpose

   Expresses SpCas9 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9 and puromycin resistance genes
   

   Expression

   Mammalian

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

   Plasmid

   #192650

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

   Plasmid

   #194063

   Purpose

   Mammalian expression of evoCjCas9 PEmax_deltaRnH

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-PEmax_dRnH
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

   Plasmid

   #192651

   Purpose

   3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-10xGCN4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. G783A_pLenti-Tet-BattP(GA)-miRFP670-2A-iCasp9-2A-Hygro_rtTA3

   Plasmid

   #200635

   Purpose

   Negative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Promoter

   pTRE3G

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB082a

   Plasmid

   #79233

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d123
   

   Expression

   Bacterial

   Promoter

   Ppro1

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-mCherry_SNAP_SNAPV_PACT_Active

   Plasmid

   #173072

   Purpose

   Active SNAP tag at the centrosome

   Depositor

   Heidi Hehnly

   Article

   Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

   Insert

   mCherry-SNAP-SNAP-Varied-PACT
   

   Tags

   mCherry-SNAP-SNAP-Varied

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZS165 CRISPEY HH-HDV NotI

    Plasmid

    #114451

    Purpose

    Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Type

    Empty backbone

    Use

    CRISPR; Cen/ars

    Expression

    Yeast

    Promoter

    ScGAL7p

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGC-Lb12-P1Bb

    Plasmid

    #231387

    Purpose

    For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    U6-29
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-EF1alpha-scFv-p65-HSF1-Blast

    Plasmid

    #192652

    Purpose

    3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-p65-HSF1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiV2-mU6-evopreQ1

    Plasmid

    #210190

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

    Plasmid

    #175524

    Purpose

    Co-expression of HaloTag7 (golgi) and SNAPf-tag (membrane inner leaflet) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7 and Lyn11-SNAPf
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGK plasmid

    Plasmid

    #169744

    Purpose

    Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2T-CMV-evoCjCas9-ABE8e-BlastR

    Plasmid

    #194060

    Purpose

    Mammalian expression of evoCjCas9 ABE8e

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSG5-SUV39H2-FL

    Plasmid

    #89135

    Purpose

    mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    SUV39H2 (SUV39H2 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 beta1

    Plasmid

    #16042

    Depositor

    Filippo Giancotti

    Article

    Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

    Insert

    beta1 (ITGB1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. CP1041-ABEmax

    Plasmid

    #119808

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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