We narrowed to 77,030 results for: rest

1. TLCV2-Scal-T2A-EGFP-Puro

   Plasmid

   #234949

   Purpose

   Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TLCV2-ApaLI-T2A-EGFP-Puro

   Plasmid

   #234948

   Purpose

   Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGK-DTAG-NTERM-GFP

   Plasmid

   #185775

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-dCas9/pCMV-7.1

   Plasmid

   #47948

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A (catalyticall…

   Promoter

   CMV

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGfa2-nLac

   Plasmid

   #53126

   Purpose

   Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.

   Depositor

   Michael Brenner

   Article

   Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.

   Insert

   Gfa2 (GFAP Human)
   

   Use

   Mouse Targeting

   Tags

   LacZ, NLS, and mP1

   Expression

   Mammalian

   Mutation

   contains -2163 to +47 (the human gfa2 segment), w…

   Promoter

   human GFAP

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGK-HALO-CTERM-GFP

   Plasmid

   #185777

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, Halo

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGK-HALO-NTERM-GFP

   Plasmid

   #185772

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, Halo

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-EGFP-eDHFR(69K6)-MCS

   Plasmid

   #172100

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-20aa
   

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

   Plasmid

   #121966

   Purpose

   Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   superfold GFP-GB1-NLS-iLID

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CRISPRv2-sgNTC13-hygro

    Plasmid

    #231988

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEM5Z(+)-EGFP

    Plasmid

    #65206

    Purpose

    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Expression

    Bacterial and Mammalian

    Mutation

    Restriction enzyme recognition sites added either…

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCherryGFP-CoV2-Inframe-stop

    Plasmid

    #177620

    Purpose

    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CRISPRv2-sgNTC49-puro

    Plasmid

    #231989

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. ALFA-bArr1

    Plasmid

    #201495

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    ALFA-bArr1 (ARRB1 Human)
    

    Tags

    ALFA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-SC4

    Plasmid

    #236254

    Purpose

    AAV-SC4 capsid which targets peripheral nerves in mice

    Depositor

    Casey Maguire

    Article

    Abou Haidar et al Gene Ther. 2025 Jun 10. doi: 10.1038/s41

    Inserts

    rep/cap AAV9 VP1 with GATPSTQ peptide insert
    rep
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_Ef1a:PE2-P2A-GFP

    Plasmid

    #184445

    Purpose

    Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and Ef1A

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER21-SOX4

    Plasmid

    #51304

    Purpose

    Expression of SOX4 after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    SRY (sex determining region Y)-box 4 (SOX4 Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Mutation

    full length ORF

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-fDIO-pAce-Kv2.1PR

    Plasmid

    #195535

    Purpose

    Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAce-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Ace-mNeon 78K, 81D, 92N, 178F; SY linker

    Promoter

    CAG

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv2 sgIRF3#5

    Plasmid

    #127642

    Purpose

    Knock-out of human IRF3

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 sgRNA (IRF3 Human)
    

    Use

    Lentiviral

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid

    #181844

    Purpose

    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-AKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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