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pRS305:Met3p-mCherry-Tub1

Plasmid

#37553

Depositor

Wei-Lih Lee

Article

Markus et al Curr Biol. 2009 Feb 10;19(3):196-205. Ep

Insert

Met3p-mCherry-Tub1

Expression

Bacterial

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only
pJW1667

Plasmid

#112041

Purpose

CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ERG5

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1670

Plasmid

#112044

Purpose

CEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

OLE1

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

P319T

Promoter

GAL1

Available Since

May 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1668

Plasmid

#112042

Purpose

CEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

FET3

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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Tubp-Gal80 in pCaSpeR4

Plasmid

#17748

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL80 (GAL80 Budding Yeast)

Tags

SV40 polyA and Tubulin 1alpha promoter

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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PAP-delta10-cHIS

Plasmid

#21716

Depositor

Andrew Bohm

Article

Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

Insert

polyA polymerase (PAP1 Budding Yeast)

Tags

HIS

Expression

Bacterial

Mutation

30 amino acids off the c terminus

Available Since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only

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SJZ9-amdS

Plasmid

#228261

Purpose

Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

amdS

Expression

Yeast

Promoter

ENO2 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ5-HPH

Plasmid

#228258

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

hph

Expression

Yeast

Promoter

SSA1 S.c.

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Expression

Yeast

Promoter

GPM1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ4-NAT

Plasmid

#228257

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

nat1

Expression

Yeast

Promoter

FBA1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLS410

Plasmid

#239123

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCEV-G1-Km

Plasmid

#46813

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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HSP104-b/Leu(WT)

Plasmid

#1156

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Tags

GAL1:10 promoter

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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VT028327-FRT10-STOP-FRT10-GAL4

Plasmid

#232829

Purpose

Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBPhsFlp1

Plasmid

#32148

Purpose

Expresses FLP recombinase in Drosophila

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

hsFlp1

Expression

Insect

Mutation

Aspartic Acid at Amino Acid 5

Promoter

Hsp70Bb promoter

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pFA6a-HBH-kanMX6

Plasmid

#26872

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag

Use

Pcr tagging yeast

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pJJB1402

Plasmid

#218616

Purpose

Express Pex15, Pex22, Pex4, Pex1, and Pex6

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex15

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMBI

Plasmid

#17816

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pSEVA6213S

Plasmid

#122094

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Promoter

Pem7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGTy1mhis3-AI or pGTy1his3-AI

Plasmid

#62228

Purpose

A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

Depositor

David Garfinkel

Article

Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

Insert

Ty1H3 retrotransposon

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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