We narrowed to 21,966 results for: EMB

1. pTH732-2µ-RLuc/staCFLuc

   Plasmid

   #40607

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   The last three codons of the full-length Firefly …

   Promoter

   ADH1 and TDH3

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Solo-mStayGold-MYO1C

   Plasmid

   #227306

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NKP90 RFP (787)

   Plasmid

   #62001

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   NKP90 (TMEM126A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   pgk

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-MCS-eDHFR(69K6)-EGFP

   Plasmid

   #172101

   Purpose

   Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   22aa-eDHFR(69K6)-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQFlag-USP11 CS puroR

   Plasmid

   #46748

   Purpose

   Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C318S--catalytically inactive

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. hnRNPA2_LC_nocharge

   Plasmid

   #139118

   Purpose

   express hnRNPA2 LC with no charged residues

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   all charged residues are changed to S/Q except fo…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKK-TEV-3XFLAG

   Plasmid

   #105784

   Purpose

   Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Stargazin-dCherry-FRB

   Plasmid

   #172444

   Purpose

   Expression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRB

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Cacng2 (Cacng2 Mouse)
   

   Use

   Tags

   dCherry-HA-FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. USPL1cat-pETM30

   Plasmid

   #85768

   Purpose

   bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514) (USPL1 Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   catalytic domain (aa 212-514)

   Promoter

   T7-lac

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. RepairTemplate_Smc4-mAID-HaloTAG

    Plasmid

    #217658

    Purpose

    Repair template to endogenously tag SMC4 with mAID-Halo Tag

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    SMC4-mAID-HaloTag (SMC4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

    Plasmid

    #79259

    Purpose

    Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    DmeIF4G (eIF4G1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    V5-His6 and lambdaN-HA

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. USPL1cat-pEYFP_C1

    Plasmid

    #85766

    Purpose

    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-HA-TEV

    Plasmid

    #105769

    Purpose

    Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    HA-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-TEV-HA

    Plasmid

    #105785

    Purpose

    Expression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-PM-InPAkt

    Plasmid

    #181915

    Purpose

    Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

    Insert

    pm-InPAkt
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Contains the following mutations with respect to …

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. NET50 pmRFP-N2 (489)

    Plasmid

    #61983

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET50 (DHRS7 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

17. NET97 pmRFP-N2 (641)

    Plasmid

    #61994

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    AADACL1 (NCEH1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pKK-TEV-FLAG

    Plasmid

    #105783

    Purpose

    Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-PBD H538A/K540M

    Plasmid

    #60879

    Purpose

    Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA 365-653 H538A/K540M mutant

    Depositor

    Trina Schroer

    Article

    Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

    Insert

    Polo-like kinase 1 (PLK1 Human, BC01486)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H538A/K540M

    Promoter

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

20. NET37 pmRFP-N2 (450)

    Plasmid

    #61980

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET37 (KIAA1161 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only