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TVBB C-term-mScarlet-Puro

Plasmid

#169220

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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HRAS

Plasmid

#55653

Purpose

expression of mouse HRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

HRAS (Hras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen-Puro

Plasmid

#169228

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK3-flag

Plasmid

#15496

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK3 (DKK3 Human)

Tags

flag

Expression

Mammalian

Mutation

See note below

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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Human USP7_H456A-3X FLAG

Plasmid

#225335

Purpose

Lentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

USP7 (USP7 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

H456A

Promoter

EF1a

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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ZFP36L2

Plasmid

#156149

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZFP36L2 (ZFP36L2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK1-flag

Plasmid

#15494

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK1 (DKK1 Human)

Tags

flag

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pCWXPGR-pTF-Wnt3a

Plasmid

#114287

Purpose

TET-inducible expression of Wnt3a

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149054)

Plasmid

#76712

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9-HE-Geminin

Plasmid

#109402

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

HE-Geminin

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-C3-PA-10H-SIII

Plasmid

#175443

Purpose

HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Tags

3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-HA

Plasmid

#120171

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF1A*-strep

Plasmid

#120165

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 1A (Kif1a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF1A: 1-405 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp4

Plasmid

#168817

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp4 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223 MERS-CoV NSP6_stop

Plasmid

#168633

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP6 (orf1ab )

Use

Other

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 MERS-CoV NSP3_nostop

Plasmid

#168608

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP3 (orf1ab )

Use

Other

Available Since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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PIK3C2B gRNA (BRDN0001149112)

Plasmid

#76713

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART1

Plasmid

#133247

Purpose

Express FLAG-tagged MCART1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART1 (SLC25A51 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells and…

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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TRPM7 gRNA (BRDN0001147821)

Plasmid

#76112

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM7 (TRPM7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ZNF217_pLX307

Plasmid

#98384

Purpose

Lentiviral expression of ZNF217

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ZNF217 (ZNF217 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-BAP1-HA

Plasmid

#154020

Purpose

Mammalian Expression of HA-tagged Wild-Type BAP1

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLgw RFC1-V5-EcoDam

Plasmid

#59205

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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mCh-KIF17*-strep

Plasmid

#120167

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 17 (KIF17 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF17: 1-509 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF13A*-strep

Plasmid

#120166

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 13A (KIF13A Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF13A: 1-639 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(deltaNIC)

Plasmid

#110191

Purpose

Expression of mouse MeCP2 (deltaNIC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini. Intervening region replace…

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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ZCCHC6

Plasmid

#156140

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZCCHC6 (TUT7 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-SBP-VAP-A*

Plasmid

#120175

Purpose

Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

VAMP associated protein A (VAPA Human)

Tags

EGFP and SBP

Expression

Mammalian

Mutation

Truncated VAP-A: 118-249

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_SARS-CoV-1-Spike

Plasmid

#168864

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-Spike (S )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PKD1 gRNA (BRDN0001149544)

Plasmid

#76841

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKD1 (PKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-puro-tetON-shRFP

Plasmid

#110940

Purpose

Tet-inducible shRNA targeting RFP

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shRFP

Use

Lentiviral and RNAi

Promoter

H1/TO

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239024

Purpose

Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin2-SEC61B (SEC61B Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-20b-M-Ntx-S1

Plasmid

#58716

Purpose

Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

Methionine

Expression

Bacterial

Mutation

Changed Asparagine 1 to Serine

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pB-Gaolf-Integration

Plasmid

#129456

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

G alpha olf (Gnal Mouse)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

Plasmid

#164827

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Promoter

miniCMV and pGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-OC43-nsp12

Plasmid

#168949

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp12 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PPIB

Plasmid

#155838

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPIB (PPIB Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLOW31

Plasmid

#172698

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-3

Available Since

Aug. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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