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Plasmid#188945PurposePlasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoterDepositorInsertdrl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
UseTol2 destination vector (attr4-r3)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGREAT18
Plasmid#170906PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP-EGFP-ULK1(1-828)
Plasmid#38198DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationdeleted amino acids 829-1051PromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGREAT17
Plasmid#170905PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2R+pNOS_Red-F_NOSt
Plasmid#170888PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT4
Plasmid#170892PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUS
Plasmid#193865PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.DepositorInsertAtWUS E. Coli Codon Optimized
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGREAT11
Plasmid#170899PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT15
Plasmid#170903PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT16
Plasmid#170904PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT19
Plasmid#170907PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT20
Plasmid#170908PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT21
Plasmid#170909PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT22
Plasmid#170910PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT23
Plasmid#170911PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT24
Plasmid#170912PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT25
Plasmid#170913PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT27
Plasmid#170915PurposeGoldenBraid Double Transcriptional Unit - pAtNR1_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtNR1Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT3
Plasmid#170891PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT5
Plasmid#170893PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT6
Plasmid#170894PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT7
Plasmid#170895PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT8
Plasmid#170896PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT9
Plasmid#170897PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT10
Plasmid#170898PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT12
Plasmid#170900PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT13
Plasmid#170901PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT14
Plasmid#170902PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2+pNOS_Red-F_NOSt
Plasmid#170887PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Forward orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mGCN2-FLAG-IP
Plasmid#101794PurposeRetroviral vector encoding C-terminally FLAG tagged mouse GCN2DepositorInsertGcn2 (Eif2ak4 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mPKR-FLAG-IG
Plasmid#101792PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PKRDepositorInsertPkr (Eif2ak2 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP spGFP-ERGIC53
Plasmid#38270DepositorInsertERGIC-53 (LMAN1 Human)
UseRetroviralTagsEGFP with Calreticulin signal peptide at N-termin…ExpressionMammalianMutationdeleted signal peptide (1-30 amino acids)PromoterAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mGLIS1
Plasmid#33273PurposeRetroviral expression of mouse Glis1DepositorInsertGLIS1, Gateway casette A (Glis1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mPERK-FLAG-IP
Plasmid#101793PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PERKDepositorInsertPerk (Eif2ak3 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mHRI-FLAG-IP
Plasmid#101791PurposeRetroviral vector encoding C-terminally FLAG tagged mouse HRIDepositorInsertHri (Eif2ak1 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXsIP GFP-VMP1
Plasmid#117417PurposeExpresses VMP1 tagged with GFP in mammalian cellsDepositorInsertVMP1 (VMP1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro Vps34-GFP
Plasmid#38268DepositorInsertPhosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-2xAM
Plasmid#92220PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sitesDepositorInsertsSp-dCas9-2xAM tag
gRNA to be inserted into Bbs I sites
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6Available sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)
Plasmid#194222PurposeMammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptorsDepositorInsertSynNotch-GAL4 DBD-VP64 AD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only