We narrowed to 17,579 results for: Mpl

1. pCSN061

   Plasmid

   #101725

   Purpose

   Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-REDD1 77-232 (#426)

   Plasmid

   #99921

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 77-232

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. shTCF7L2 # 2

   Plasmid

   #42558

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shTCF7L2 (TCF7L2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-SPORT6-RNF114-RINGmut

   Plasmid

   #69922

   Purpose

   Expresses mutant myc-RNF114 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

   Insert

   RNF114 (RNF114 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C29A, C32A

   Promoter

   CMV

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. GST-PRDM16 1039-1176

   Plasmid

   #53351

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Bruce Spiegelman, Shingo Kajimura

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 1039-1176

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. GST-PRDM16 681-880

   Plasmid

   #53349

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Bruce Spiegelman, Shingo Kajimura

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 681-880

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. GST-PRDM16 455-680

   Plasmid

   #53348

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Bruce Spiegelman, Shingo Kajimura

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 455-680

   Promoter

   tac

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pRN3P-HA-Suv4-20h1

   Plasmid

   #86689

   Purpose

   For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

   Insert

   histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mutation

   Promoter

   T3

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1 p18 mito

   Plasmid

   #26638

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   p18 mito (LAMTOR1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pMXs-puro HA-Atg14*

    Plasmid

    #38265

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed A618T, A621T, C624A and T627C for siRNA r…

    Promoter

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pMX GFP cdc42 Q61L

    Plasmid

    #14568

    Depositor

    Joan Brugge

    Article

    Hall et al Immunity. 2006 Mar . 24(3):305-16.

    Insert

    cdc42 Q61L (CDC42 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pIK208

    Plasmid

    #65630

    Purpose

    sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Insert

    U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA_DiCre_60.F2

    Plasmid

    #60208

    Purpose

    Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
    

    Use

    Tags

    FRAP and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 2021-2112 of FRAP with a T2098L mutat…

    Promoter

    CMV

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA1 Flag ATXN1L

    Plasmid

    #33242

    Depositor

    Huda Zoghbi

    Article

    Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMSCV-Zeo-TTF-1

    Plasmid

    #75089

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    TTF-1 (NKX2-1 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild-type, full-length, without native 3' UTR

    Promoter

    Available since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSVL-mo FceRI gamma

    Plasmid

    #8372

    Depositor

    Jean Kinet

    Article

    Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

    Insert

    mouse Fc epsilon RI gamma cDNA (Fcer1g Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-GLI(-)TAD

    Plasmid

    #37113

    Depositor

    David Walterhouse

    Article

    Yoon et al J Biol Chem. 1998 Feb 6;273(6):3496-501.

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted 1,409 bp AccI fragment from 3' end o…

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pSVE Grb2 myc 49L/203R

    Plasmid

    #26087

    Depositor

    Bob Weinberg

    Article

    Egan et al Nature. 1993 May 6. 363(6424):45-51.

    Insert

    Grb2 49L/203R (GRB2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    49L/203R

    Promoter

    Available since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

19. ANKRD24-TwinStrepII

    Plasmid

    #83190

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    ANKRD24 (Ankrd24 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGS1034

    Plasmid

    #63796

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

    Insert

    CBP-3xFLAG-TEV-Protein A followed by KlLEU2
    

    Use

    E. coli cloning vector

    Tags

    CBP-3xFLAG-TEV-Protein A

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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