235,755 results

1. pYES2-Ub-M-GFP

   Plasmid

   #11952

   Depositor

   Nico Dantuma

   Article

   Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

   Insert

   Ub-M-GFP
   

   Expression

   Yeast

   Mutation

   Ubiquitin fused to N-terminus of GFP. Methionine …

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_tre_dCas9_VP160

   Plasmid

   #126031

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with VP160
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pComb3XTT

   Plasmid

   #63891

   Purpose

   Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   TT Fab
   

   Use

   Phage display

   Tags

   6xHis, HA tag, and geneIII

   Expression

   Bacterial

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pComb3XTT

   Plasmid

   #63891

   Purpose

   Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   TT Fab
   

   Use

   Phage display

   Tags

   6xHis, HA tag, and geneIII

   Expression

   Bacterial

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-ORF-V2

   Plasmid

   #73638

   Purpose

   Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-ORF-V2

   Plasmid

   #73638

   Purpose

   Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-Mito4x-GCaMP6f

   Plasmid

   #127870

   Purpose

   Fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   4 repeats of COX8 signal peptide plus GCaMP6f
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBP-Benzonase

   Plasmid

   #218192

   Purpose

   Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

   Depositor

   Cheryl Arrowsmith

   Insert

   nucA
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   April 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pET-11b_PcrA_M6_H93A

   Plasmid

   #194156

   Purpose

   Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

   Depositor

   Taekjip Ha

   Article

   Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

   Insert

   Engineered helicase PcrA_M6
   

   Tags

   6x His tag and FLAG tag

   Expression

   Bacterial

   Mutation

   H93A, C96A, N187C, C247A, L384V, L409C

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn1-FLEX-CybGam-WPRE

    Plasmid

    #207662

    Purpose

    Control for CybSEP2

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Gamillus
    

    Use

    AAV

    Mutation

    Histidine 86 and 159 to alanines, inserted gamill…

    Promoter

    hSynapsin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLumi-LgBiT

    Plasmid

    #188319

    Purpose

    Expression of LgBiT via sigma 70 promoter in E. coli

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    LgBiT
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Promoter

    sigma 70 promoter

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. At2S3:RUBY

    Plasmid

    #160906

    Purpose

    RUBY under the control of At2S3 promoter/marker for Arabidopsis transgenes

    Depositor

    Yunde Zhao

    Article

    He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

    Insert

    RUBY and At2S3 promoter
    

    Expression

    Plant

    Promoter

    At2S3

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-STAT3

    Plasmid

    #111934

    Purpose

    Expresses human STAT3 fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Signal transducer and activator of transcription 3 (STAT3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV5-Flag-RhoA wt

    Plasmid

    #11750

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    RhoA (RHOA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pCMV5-Flag-RhoA wt

    Plasmid

    #11750

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    RhoA (RHOA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

20. Human CRISPRi sgRNA library Dolcetto in backbone XPR_050

    Pooled Library

    #1000000114

    Purpose

    Genome-wide CRISPR gRNA pooled library for inhibition of human genes

    Depositor

    John Doench, David Root

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

21. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

    Pooled Library

    #83994

    Purpose

    Mouse CRISPRi Pooled Library targeting membrane proteins

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

23. mTurquoise-Rab5a-7

    Plasmid

    #55578

    Purpose

    Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mTurquoise

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

24. mTurquoise-Rab5a-7

    Plasmid

    #55578

    Purpose

    Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mTurquoise

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. pAAV-hsyn-GRAB_rDA1h (AAV9)

    Viral Prep

    #140557-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1h (#140557). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1h plasmid DNA. Synapsin-driven expression of red fluorescent, high affinity dopamine sensor GRAB_rDA1h. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Promoter

    Syn

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

26. JDS246

    Plasmid

    #43861

    Purpose

    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

    Depositor

    Keith Joung

    Insert

    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. JDS246

    Plasmid

    #43861

    Purpose

    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

    Depositor

    Keith Joung

    Insert

    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mScarlet-I-PXN

    Plasmid

    #112956

    Purpose

    In vivo visualization of focal adhesions (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    PXN
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pT3-EF1A-mTert

    Plasmid

    #162555

    Purpose

    Overexpression of Tert

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of Tert (Tert Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

    Plasmid

    #192616

    Purpose

    AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    FLEX-RIboL1-jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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