We narrowed to 17,411 results for: EMB

1. pJET-50-DExogron-mCherry-R11b

   Plasmid

   #179904

   Purpose

   Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMKRQ_11_DExCon-mNeonGreen-R25

   Plasmid

   #179896

   Purpose

   Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB25, member RAS oncogene family (RAB25 Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJET-23-DExCon-mCherry-R11b

   Plasmid

   #179898

   Purpose

   Donor with homologous arms for Rab11b to knock in DExCon-mCherry module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJET-67-DExCon-mNeonGreen-R11b

   Plasmid

   #179899

   Purpose

   Donor with homologous arms for Rab11b to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJET-59-DExCon-mTagBFP2-R25

   Plasmid

   #179900

   Purpose

   Donor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB25, member RAS oncogene family (RAB25 Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mTagBFP2

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

   Plasmid

   #148402

   Purpose

   Insect Expression of DmNot1_1674-1962-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   DmNot1_1674-1962-dsRNAres (Not1 Fly)
   

   Expression

   Insect

   Mutation

   2 silent mutations compared to the sequence given…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.U6gT28.4.hSyn.flex.H2B.RFP

   Plasmid

   #170386

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.U6gT28.3.hSyn.flex.H2B.RFP

   Plasmid

   #170374

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.U6gT28.13.hSyn.flex.H2B.RFP

   Plasmid

   #170376

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHA853 - mec-4p::Fynempty

    Plasmid

    #139210

    Purpose

    Control vector for Fyn kinase expression in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Expression

    Worm

    Mutation

    deletion and frame shift to prevent Fyn expression

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHA850 - osm-10p::FynY531F

    Plasmid

    #139207

    Purpose

    Express activated Fyn kinase in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Expression

    Worm

    Mutation

    Y531F

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHA851 - osm-10p::Fynempty

    Plasmid

    #139208

    Purpose

    Control vector for Fyn kinase expression in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Expression

    Worm

    Mutation

    deletion and frame shift to prevent Fyn expression

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUS_LC_withR

    Plasmid

    #139128

    Purpose

    express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Expression

    Bacterial

    Mutation

    add arginines

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. hnRNPA2_LC_nocharge_wR

    Plasmid

    #139119

    Purpose

    express hnRNPA2 LC with no charged residues with R added back

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Expression

    Bacterial

    Mutation

    remove all K, most D

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUS_LC_charge

    Plasmid

    #139126

    Purpose

    express FUS LC with an hnRNPA2 LC-like charge patterning

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Expression

    Bacterial

    Mutation

    Add R, K, D to make FUS LC have similar charge pa…

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-Miro1 (1-177 fused to 562-618)

    Plasmid

    #127627

    Purpose

    First GTPase domain of human Miro1 fused to C-terminus

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    1-177 fused to 562-618

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Oct4deltaPE-53BP1mCherry

    Plasmid

    #141122

    Purpose

    Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

    Depositor

    John Schimenti

    Article

    Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

    Insert

    Fragment of human 53BP1 (TP53BP1 Human)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. NKP91 GFP (1034)

    Plasmid

    #62023

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    Tmem41A (TMEM41A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAV10.F3.loxP

    Plasmid

    #63200

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV10.HO5.loxP

    Plasmid

    #63206

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge