We narrowed to 11,355 results for: SHA

1. pInducer20 CARD8-HA PAM R394E

   Plasmid

   #169987

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, R394E

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pInducer20 CARD8-HA PAM F405G

   Plasmid

   #169988

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, F405G

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pInducer20 CARD8-HA PAM F370G

   Plasmid

   #169986

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, F370G

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pInducer20 CARD8-HA PAM E274R

   Plasmid

   #169984

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, E274R

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR221 CARD8 FIIND F405G + S297A

   Plasmid

   #169974

   Purpose

   pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   S297A, F405G

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR221 CARD8 FIIND E274R + S297A

   Plasmid

   #169968

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   S297A, E274R

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221 CARD8 FIIND R394E + S297A

   Plasmid

   #169972

   Purpose

   pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   S297A, R394E

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221 CARD8 PAM E274R

   Plasmid

   #169977

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   PAM, E274R

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

   Plasmid

   #169955

   Purpose

   Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S297A, R394E

   Promoter

   CMV

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

    Plasmid

    #169957

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, F405G

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

    Plasmid

    #169951

    Purpose

    Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E274R, S297A

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Ir7a-T2A-QF2 HDR plasmid

    Plasmid

    #140943

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSECC-sgSIK2-A

    Plasmid

    #138670

    Purpose

    Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 mouse (Sik2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSECC-sgSIK2-B

    Plasmid

    #138671

    Purpose

    Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 mouse (Sik2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNCST-AvicFP3

    Plasmid

    #129506

    Purpose

    Expresses AvicFP3 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-myc-HLA-B15-VN

    Plasmid

    #135514

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*15:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*15:01 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4-myc-HLA-B57-VN

    Plasmid

    #135515

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*57:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*57:1 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-myc-H2-Dd (Y84C/C121S/A139C)

    Plasmid

    #136063

    Purpose

    Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Y84C/C121S/A139C

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-myc-HLA-B8-VN

    Plasmid

    #135511

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*08:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*08:01 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tet-pLKO shMKP7.v2 puro

    Plasmid

    #87795

    Purpose

    Expresses an inducible short hairpin targeting human MKP7 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP7 (DUSP16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge