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pFF838

Plasmid

#61457

Purpose

aTc-inducible SCRIBE_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_lacZ(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-18a

Plasmid

#61326

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pICSL50004

Plasmid

#50316

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mCherry variant of RFP (Discosoma sp.)

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH51288

Plasmid

#50269

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EcMBP165-cpGFP.PPYF.pRSET

Plasmid

#33372

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

MBP based Maltose Biosensor

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCAT206

Plasmid

#134883

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT205

Plasmid

#134882

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB405-TIC21-sGFP

Plasmid

#213865

Purpose

Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

Insert

chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-353)-GCN4-L-mCherry

Plasmid

#193715

Purpose

Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)

Tags

mCherry

Expression

Mammalian

Mutation

hsKIF5B(aa1-353); mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc p31Comet

Plasmid

#59833

Purpose

Allows the integration of myc p31Comet in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Westhorpe et al J Cell Sci. 2011 Nov 15;124(Pt 22):3905-

Insert

p31 (MAD2L1BP Human)

Tags

Myc

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-MUTED

Plasmid

#164629

Purpose

Expresses MUTED in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

MUTED (BLOC1S5 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-hCD2-CD14 (4R)

Plasmid

#133062

Purpose

Retroviral expression of mouse CD14 mutant "4R" deficient in LPS binding

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

CD14 (4R) (Cd14 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

CD14 allele defective for LPS binding (mutations …

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AMPKsub-YPet-NES

Plasmid

#84635

Purpose

Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub-YPet-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pExo1

Plasmid

#53357

Purpose

Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.

Depositor

Lingchong You

Article

Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10

Inserts

BlaMs
hlyAs

Promoter

p(lac/ara-1)

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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4X PRE TK luc

Plasmid

#206159

Purpose

Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

4X PRE TK luciferase

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-TRE-EB3N-VVDfast-mCl3-iLID

Plasmid

#190169

Purpose

Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-mClover3-iLID

Use

Lentiviral

Tags

-VVDfast-mClover3-iLID

Mutation

1-189 aa from EB3

Promoter

TRE

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1 FLCN (WT) FLAG GFP

Plasmid

#164978

Purpose

Lentiviral vector for FLCN expression in mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FLCN (FLCN Human)

Use

Lentiviral

Tags

1xFLAG-GFP

Expression

Mammalian

Promoter

UBC

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

Plasmid

#156445

Purpose

For transient expression of mouse RAD21 (SCC1) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse RAD21 (Rad21 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-sfGFP-3xP3-RFP

Plasmid

#127557

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sfGFP

Use

CRISPR

Expression

Insect

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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