We narrowed to 13,167 results for: Mpl;

1. pWzyE-GST-distrepII

   Plasmid

   #196666

   Purpose

   Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   GST and TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWzyE-strep-mClover3

   Plasmid

   #196668

   Purpose

   Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   TEV-cleavable di-strep tag II and mClover3

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

   Plasmid

   #217514

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-C1-BBS9

   Plasmid

   #218717

   Purpose

   Expresses N-terminally EGFP-tagged BBS9 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   BBS9 (BBS9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A455T (g1876a)

   Promoter

   CMV

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMRX-IP-hWIPI2B-3×FLAG

   Plasmid

   #215510

   Purpose

   Expresses FLAG tagged human WIPI2B.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)
   

   Use

   Retroviral

   Tags

   3×FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21b-effector-ncRNA-rrt-His8

   Plasmid

   #194089

   Purpose

   Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 effector-ncRNA-rrt
   

   Use

   Tags

   His8

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VF-Sensor

   Plasmid

   #208927

   Purpose

   in vitro RSK-Sensor for luminescent detection of RSK-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   SOS1 (SOS1 Human)
   

   Use

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PDE4B-Sensor

   Plasmid

   #208929

   Purpose

   in vitro JNK-Sensor for luminescent detection of JNK-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
   

   Use

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NSP4-EGFP

   Plasmid

   #210378

   Purpose

   Expresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   NSP4 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NSP2-Flag

    Plasmid

    #210344

    Purpose

    Expresses SARS-CoV-2 NSP2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Drosophila Usp46-V5

    Plasmid

    #217620

    Purpose

    Mammalian expression of V5 tagged Drosophila Usp46

    Depositor

    Ethan Lee

    Article

    Spencer et al Nat Commun. 2023 Oct 5;14(1):6174. doi:

    Insert

    USP46 (Usp12-46 Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Human USP46

    Plasmid

    #217615

    Purpose

    Mammalian expression of USP46

    Depositor

    Ethan Lee

    Article

    Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

    Insert

    USP46 (USP46 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Halotag-6xHp

    Plasmid

    #214414

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to Halotag

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CFAST-Halotag-6xHp

    Plasmid

    #214416

    Purpose

    Lipid droplet localization of CFAST fused to Halotag-6xHp

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    deletion
    

    Use

    Tags

    CFAST-Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mApple-1xHp

    Plasmid

    #214409

    Purpose

    Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p667-UBC-MAFG-V5-miniTurbo

    Plasmid

    #207818

    Purpose

    C-terminal tagged MAFG for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    MAFG-V5-miniTurbo
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Expression

    Mutation

    Promoter

    UBC

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NSP3C-mCherry

    Plasmid

    #210377

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    NSP3C (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p663-UBC-miniTurbo-V5-MAFG

    Plasmid

    #207817

    Purpose

    N-terminal tagged MAFG for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-MAFG
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p663-UBC-miniTurbo-V5-ZNF746

    Plasmid

    #207819

    Purpose

    N-terminal tagged ZNF746 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-ZNF746 (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TLNRD1_35-345_eGFP

    Plasmid

    #208881

    Purpose

    Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 (TLNRD1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acid sequence 35-345

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mito-PDCD10-mScarlet-I

    Plasmid

    #208887

    Purpose

    Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM3 (PDCD10 Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    PDCD10 targeted to the mitonchondria

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TLNRD1_5H_eGFP

    Plasmid

    #208861

    Purpose

    Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 5 helix bundle (TLNRD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted amino acid 142-273

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SEC61B-2Strep

    Plasmid

    #210375

    Purpose

    Expresses SEC61B fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SEC61B (SEC61B Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TMEM106B-2Strep

    Plasmid

    #210360

    Purpose

    Expresses TMEM106B fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    TMEM106B (TMEM106B Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-Cas9-NS-A

    Plasmid

    #207823

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-Cas9-NS-B

    Plasmid

    #207824

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCW57.1-ZNF746-C571A

    Plasmid

    #207826

    Purpose

    Dox-inducible expression of ZNF746-C571A

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746-C571A (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C571A

    Promoter

    TRE

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p667-UBC-ZNF746-V5-miniTurbo

    Plasmid

    #207820

    Purpose

    C-terminal tagged ZNF746 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746-V5-miniTurbo (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cav2.2 BBS exon37a pCAGGS

    Plasmid

    #206086

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 1533_pAAV-CB-AcGFP-BirA-HA

    Plasmid

    #208201

    Purpose

    Control for FKBP12-BirA construct

    Depositor

    Susan Hamilton

    Article

    Lee et al Commun Biol. 2023 Sep 14;6(1):942. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Chicken beta actin

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GFP Cav2.2 HA pCAGGS

    Plasmid

    #206054

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GFP Cav2.2 HA pcDNA3

    Plasmid

    #206053

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-BCR-ABL-p210-BT-FLAG

    Plasmid

    #205621

    Purpose

    Expression of BCR-ABL oncogene in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

    Insert

    BCR-ABL oncogene, p210 isoform b3a2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    deleted BT domain

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 1BR9-AtWUSSTOP

    Plasmid

    #202053

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized (WUS Mustard Weed)
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA5-FRT-TO-nsp7

    Plasmid

    #201025

    Purpose

    Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet-ON

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBMN HA-ATG13(Y118D)

    Plasmid

    #188638

    Purpose

    For expression of human ATG13(Y118D)

    Depositor

    Michael Lazarou

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed tyrosine 118 to aspartic acid

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBMN HA-ATG13(W50D)

    Plasmid

    #188639

    Purpose

    For expression of human ATG13(W50D)

    Depositor

    Michael Lazarou

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed tryptophan 50 to aspartic acid

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-20b_HtrA2(delta(133))_S306A_6His

    Plasmid

    #197975

    Purpose

    E. coli expression of HtrA2(delta133)-6His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    delta(1-133), S306A

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGBT9-GGA2-GAE

    Plasmid

    #199175

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    GGA2-GAE domain (GGA2 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Contains Lys 545 Gln substitution

    Promoter

    ADH1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGBT9-GGA3-GAE

    Plasmid

    #199176

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    GGA3-GAE domain (GGA3 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge